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Literature summary for 2.3.1.48 extracted from

  • Lau, O.D.; Courtney, A.D.; Vassilev, A.; Marzilli, L.A.; Cotter, R.J.; Nakatani, Y.; Cole, P.A.
    P300/CBP-associated factor histone acetyltransferase processing of a peptide substrate. Kinetic analysis of the catalytic mechanism (2000), J. Biol. Chem., 275, 21953-21959.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of the catalytic domain of PCAF protein, amino acid residues 493-658, in Escherichia coli BL21 (DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
bisubstrate analogue histone H3-peptide-coenzyme A potent inhibitor, competitive versus acetyl-CoA, non-competitive versus histone H3-peptide Homo sapiens
desulfo-coenzyme A dead-end inhibitor, noncompetitive versus histone H3-peptide and competitive versus actyl-CoA Homo sapiens
histone H3-peptide mutant K14A dead-end inhibitor analogue, mutant histone H3 -peptide consisting of amino acid residues 3-20 K14A Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Homo sapiens
0.046
-
acetyl-CoA PCAF catalytic domain Homo sapiens
0.05
-
histone H3-peptide PCAF catalytic domain Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + histone Homo sapiens the acetyl groups function as signals for interaction of histones with other regulatory proteins, chromatin remodeling CoA + acetylhistone
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
PCAF protein
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged catalytic domain of PCAF protein from E. coli Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine ternary complexed mechanism for PCAF and Gnc5 Homo sapiens
acetyl-CoA + [protein]-L-lysine = CoA + [protein]-N6-acetyl-L-lysine PCAF and Gcn5 protein, fully ordered Bi-Bi kinetic mechanism with acetyl-CoA binding before histone H3 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + histone
-
Homo sapiens CoA + acetylhistone
-
?
acetyl-CoA + histone the acetyl groups function as signals for interaction of histones with other regulatory proteins, chromatin remodeling Homo sapiens CoA + acetylhistone
-
?
acetyl-CoA + histone H3 Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain Homo sapiens CoA + acetylhistone H3
-
?
acetyl-CoA + histone H3 peptide Lys14 of histone H3 and a peptide containing Lys14 thereof are the preferred substrates for Gnc5 and PCAF protein, as well as Gnc5 and PCAF catalytic domain Homo sapiens CoA + acetylhistone H3 peptide
-
?
acetyl-CoA + histone H3-peptide
-
Homo sapiens CoA + acetylhistone H3-peptide
-
?
acetyl-CoA + histone H3-peptide
-
Homo sapiens CoA + acetylhistone H3 -peptide
-
?
additional information Gcn5 and PCAF protein are transcription cofactors Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
HAT
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
PCAF protein, assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0117
-
acetyl-CoA PCAF protein Homo sapiens
0.0117
-
histone H3-peptide PCAF protein Homo sapiens
0.0467
-
acetyl-CoA PCAF catalytic domain Homo sapiens
0.0467
-
histone H3-peptide PCAF catalytic domain Homo sapiens
0.0667
-
histone H3-peptide PCAF catalytic domain Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens
8
-
PCAF protein Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
additional information
-
Homo sapiens