Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Brij-58 | no inhibition | Zea mays | |
Cu2+ | 5 mM, enzyme form B | Zea mays | |
dimethyl sulfoxide | irreversible at 2% v/v | Zea mays | |
ethanol | irreversible at 2% v/v | Zea mays | |
Fe2+ | 5 mM, enzyme form B | Zea mays | |
Isopropanol | irreversible at 2% v/v | Zea mays | |
Triton X-100 | no inhibition | Zea mays | |
Zn2+ | 5 mM, enzyme form B | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | enzyme form B | Zea mays | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000 + 1 * 50000, enzyme form B, SDS-PAGE | Zea mays |
50000 | - |
1 * 45000 + 1 * 50000, enzyme form B, SDS-PAGE | Zea mays |
90000 | - |
enzyme form B, gel filtration | Zea mays |
Organic Solvent | Comment | Organism |
---|---|---|
dimethyl sulfoxide | irreversible inactivation at 2% v/v | Zea mays |
Ethanol | irreversible inactivation at 2% v/v | Zea mays |
isopropanol | irreversible inactivation at 2% v/v | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Zea mays | - |
enzyme form B | - |
Zea mays Cuzco | - |
- |
- |
Purification (Comment) | Organism |
---|---|
enzyme form B | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | i.e. embryo | Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Zea mays |
0.0057 | - |
purified enzyme form B | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 acetyl-CoA + histone H4 | enzyme form B | Zea mays | 2 CoA + diacetylhistone H4 | mono- and diacetylated products | ? | |
2 acetyl-CoA + histone H4 | enzyme form B | Zea mays Cuzco | 2 CoA + diacetylhistone H4 | mono- and diacetylated products | ? | |
acetyl-CoA + histone | - |
Zea mays | CoA + acetylhistone | - |
? | |
acetyl-CoA + histone | - |
Zea mays Cuzco | CoA + acetylhistone | - |
? | |
acetyl-CoA + histone H3 | enzyme form B, very low activity | Zea mays | CoA + acetylhistone H3 | - |
? | |
acetyl-CoA + histone H3 | enzyme form B, very low activity | Zea mays Cuzco | CoA + acetylhistone H3 | - |
? | |
acetyl-CoA + histone H4 | enzyme form B | Zea mays | CoA + acetylhistone H4 | mono- and diacetylated products | ? | |
acetyl-CoA + histone H4 | enzyme form B | Zea mays Cuzco | CoA + acetylhistone H4 | mono- and diacetylated products | ? |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 45000 + 1 * 50000, enzyme form B, SDS-PAGE | Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme form B | Zea mays |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme is highly unstable during purification, solubilization at -10°C | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | 8.5 | enzyme form B | Zea mays |