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Literature summary for 2.3.1.48 extracted from
- Purification and characterization of the cytoplasmic histone acetyltransferase B of maize embryos (1996), FEBS Lett., 386, 75-81.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Brij-58 | no inhibition | Zea mays |  |
| Cu2+ | 5 mM, enzyme form B | Zea mays | |
| dimethyl sulfoxide | irreversible at 2% v/v | Zea mays | |
| ethanol | irreversible at 2% v/v | Zea mays | |
| Fe2+ | 5 mM, enzyme form B | Zea mays | |
| Isopropanol | irreversible at 2% v/v | Zea mays | |
| Triton X-100 | no inhibition | Zea mays | |
| Zn2+ | 5 mM, enzyme form B | Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | enzyme form B | Zea mays | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 45000 | - |
1 * 45000 + 1 * 50000, enzyme form B, SDS-PAGE | Zea mays |
| 50000 | - |
1 * 45000 + 1 * 50000, enzyme form B, SDS-PAGE | Zea mays |
| 90000 | - |
enzyme form B, gel filtration | Zea mays |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| dimethyl sulfoxide | irreversible inactivation at 2% v/v | Zea mays |
| Ethanol | irreversible inactivation at 2% v/v | Zea mays |
| isopropanol | irreversible inactivation at 2% v/v | Zea mays |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
- |
- |
| Zea mays | - |
enzyme form B | - |
| Zea mays Cuzco | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| enzyme form B | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | i.e. embryo | Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Zea mays |
| 0.0057 | - |
purified enzyme form B | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 acetyl-CoA + histone H4 | enzyme form B | Zea mays | 2 CoA + diacetylhistone H4 | mono- and diacetylated products | ? | |
| 2 acetyl-CoA + histone H4 | enzyme form B | Zea mays Cuzco | 2 CoA + diacetylhistone H4 | mono- and diacetylated products | ? | |
| acetyl-CoA + histone | - |
Zea mays | CoA + acetylhistone | - |
? | |
| acetyl-CoA + histone | - |
Zea mays Cuzco | CoA + acetylhistone | - |
? | |
| acetyl-CoA + histone H3 | enzyme form B, very low activity | Zea mays | CoA + acetylhistone H3 | - |
? | |
| acetyl-CoA + histone H3 | enzyme form B, very low activity | Zea mays Cuzco | CoA + acetylhistone H3 | - |
? | |
| acetyl-CoA + histone H4 | enzyme form B | Zea mays | CoA + acetylhistone H4 | mono- and diacetylated products | ? | |
| acetyl-CoA + histone H4 | enzyme form B | Zea mays Cuzco | CoA + acetylhistone H4 | mono- and diacetylated products | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 45000 + 1 * 50000, enzyme form B, SDS-PAGE | Zea mays |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
enzyme form B | Zea mays |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme is highly unstable during purification, solubilization at -10°C | Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.2 | 8.5 | enzyme form B | Zea mays |
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