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Literature summary for 2.3.1.41 extracted from

  • Christensen, C.E.; Kragelund, B.B.; von Wettstein-Knowles, P.; Henriksen, A.
    Structure of the human beta-ketoacyl [ACP] synthase from the mitochondrial type II fatty acid synthase (2007), Protein Sci., 16, 261-272.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
drug development the enzyme is a target for antibacterial drugs Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of the His-tagged enzyme in Escherichia coli strains XL-1 blue and M15 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant enzyme free or complexed with acyl-CoA, hanging drop vapour diffusion method, 0.002 ml of protein solution, containing 0.087 mg of protein with or without 4 mM hexanoyl-CoA, mixed with 0.002 ml of reservoir solution containing 24% w/v PEG 3350 and 0.2 M NH4Cl, 5-8 days at room temperature, rod-shaped single crystals, X-ray diffraction structure determination and analysis at 1.6 A resolution, structure modeling Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
cerulenin
-
Homo sapiens
additional information inhibitor binding structure determination Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion beta-ketoacyl [ACP] synthase from the mitochondrial type II fatty acid synthase Homo sapiens 5739
-

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NWU1 the human beta-ketoacyl [ACP] synthase from the mitochondrial type II fatty acid synthase
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strains XL-1 blue and M15 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] structure-activity analysis, reaction mechanism Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information KAS catalyzes the C2 fatty acid elongation reaction using a Cys-His-His catalytic triad Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
dimer secondary structure, a dimer with an alpha-beta-alpha-beta-alpha-thiolase fold capped by an alpha-helical region connecting the strands of the N-terminal beta-sheet, crystal structure analysis Homo sapiens

Synonyms

Synonyms Comment Organism
beta-ketoacyl [ACP] synthase
-
Homo sapiens
beta-ketoacyl-ACP synthase
-
Homo sapiens
KAS
-
Homo sapiens