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Literature summary for 2.3.1.41 extracted from

  • McGuire, K.A.; Siggaard-Andersen, M.; Bangera, M.G.; Olsen, J.G.; von Wettstein-Knowles, P.
    beta-Ketoacyl-[acyl carrier protein] synthase I of Escherichia coli: aspects of the condensation mechanism revealed by analyses of mutations in the active site pocket (2001), Biochemistry, 40, 9836-9845.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of synthase I and mutants as His-tagged proteins in Escherichia coli Escherichia coli

Protein Variants

Protein Variants Comment Organism
C163A synthase I, site-directed mutagenesis, active site mutant, no activity, but decarboxylates malonyl-[acyl-carrier-protein] Escherichia coli
C163S synthase I, site-directed mutagenesis, active site mutant, increased activity, decarboxylates malonyl-[acyl-carrier-protein] Escherichia coli
D306A synthase I, site-directed mutagenesis, active site mutant, increased activity Escherichia coli
E309A synthase I, site-directed mutagenesis, active site mutant, reduced activity Escherichia coli
H298A synthase I, site-directed mutagenesis, active site mutant, reduced activity Escherichia coli
H333A synthase I, site-directed mutagenesis, active site mutant, increased activity Escherichia coli
K328A synthase I, site-directed mutagenesis, active site mutant, reduced activity Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
[Acyl-carrier-protein] wild-type synthase I and mutants, not mutants C163S and K328A, overview Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
synthase I mutants Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
synthase I
-

Purification (Commentary)

Purification (Comment) Organism
recombinant synthase I and mutants as His-tagged protein from E. coli Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] mechanism and active site structure and function Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-[acyl-carrier protein] wild-type synthase I and mutants C163A and C163S Escherichia coli acetyl-[acyl-carrier protein] + CO2
-
?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase I Escherichia coli 3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
?

Subunits

Subunits Comment Organism
More synthase I mutants Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
assay at Escherichia coli