Cloned (Comment) | Organism |
---|---|
expression of synthase I and mutants as His-tagged proteins in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C163A | synthase I, site-directed mutagenesis, active site mutant, no activity, but decarboxylates malonyl-[acyl-carrier-protein] | Escherichia coli |
C163S | synthase I, site-directed mutagenesis, active site mutant, increased activity, decarboxylates malonyl-[acyl-carrier-protein] | Escherichia coli |
D306A | synthase I, site-directed mutagenesis, active site mutant, increased activity | Escherichia coli |
E309A | synthase I, site-directed mutagenesis, active site mutant, reduced activity | Escherichia coli |
H298A | synthase I, site-directed mutagenesis, active site mutant, reduced activity | Escherichia coli |
H333A | synthase I, site-directed mutagenesis, active site mutant, increased activity | Escherichia coli |
K328A | synthase I, site-directed mutagenesis, active site mutant, reduced activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
[Acyl-carrier-protein] | wild-type synthase I and mutants, not mutants C163S and K328A, overview | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
synthase I mutants | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
synthase I | - |
Purification (Comment) | Organism |
---|---|
recombinant synthase I and mutants as His-tagged protein from E. coli | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | mechanism and active site structure and function | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-[acyl-carrier protein] | wild-type synthase I and mutants C163A and C163S | Escherichia coli | acetyl-[acyl-carrier protein] + CO2 | - |
? | |
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | synthase I | Escherichia coli | 3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | synthase I mutants | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Escherichia coli |