Any feedback?
Literature summary for 2.3.1.41 extracted from
- Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli (1980), J. Biol. Chem., 255, 11949-11956.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dithiothreitol | required | Escherichia coli |  |
| dithiothreitol | activation | Escherichia coli | |
| EDTA | activation | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetic data of synthase I and II at different temperatures with various substrates | Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition, synthase I and II | Escherichia coli |
| 80000 | - |
synthase I, sedimentation equilibrium method | Escherichia coli |
| 85000 | - |
synthase II, sedimentation equilibrium method | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
synthases I and II | - |
| Escherichia coli | - |
K12 strains UC1, WN1 is a fabF-mutant lacking synthase II, and various mutants, overview | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| synthases I and II | Escherichia coli |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | structure | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
synthase I | Escherichia coli |
| 6.3 | - |
synthase II | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | poor substrate, synthase I | Escherichia coli | 3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | best substrate, synthase II | Escherichia coli | 3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | not cis-vaccenoyl-[acyl-carrier-protein], i.e. cis-11-octenoyl-[acyl-carrier-protein] | Escherichia coli | cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | from C-10 to C-16, e.g. cis-5-decenoyl-[acyl-carrier-protein], cis-5-dodecenoyl-[acyl-carrier-protein], cis-7-tetradecenoyl-[acyl-carrier-protein], cis-9-hexenoyl-[acyl-carrier-protein], i.e. palmitoleoyl-[acyl-carrier-protein], poor substrates | Escherichia coli | cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | i.e. palmitoyl-[acyl-carrier-protein] | Escherichia coli | 3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
| tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Escherichia coli | 3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 43000-44000, synthase I, SDS-PAGE | Escherichia coli |
| dimer | 2 * 44000-45000, synthase II, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 27 | - |
assay at | Escherichia coli |
| 37 | - |
assay at | Escherichia coli |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
