Application | Comment | Organism |
---|---|---|
diagnostics | coupled enzyme assay can be used in development and optimization of small-molecule inhibitors for antibacterial therapy | Escherichia coli K-12 |
Cloned (Comment) | Organism |
---|---|
from K-12 strain MC4100, overexpression in strain M15 | Escherichia coli K-12 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
malonyl-CoA | pH 6.8, 28°C, recombinant enzyme | Escherichia coli K-12 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + [acyl-carrier protein] | Escherichia coli K-12 | key enzyme in fatty acid synthesis | CoA + malonyl-[acyl-carrier protein] | malonyl-[acyl-carrier protein] is the key building block for de novo fatty acid synthesis | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli K-12 | - |
gene fabD | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from strain M15 | Escherichia coli K-12 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + [acyl-carrier protein] | key enzyme in fatty acid synthesis | Escherichia coli K-12 | CoA + malonyl-[acyl-carrier protein] | malonyl-[acyl-carrier protein] is the key building block for de novo fatty acid synthesis | ? | |
malonyl-CoA + [acyl-carrier protein] | transfer to the free thiol group of the phosphopantetheine arm of the [acyl-carrier protein] | Escherichia coli K-12 | CoA + malonyl-[acyl-carrier protein] | - |
? | |
additional information | development of a continuous fluorometric coupled assay method in microplates by coupling of the enzyme to alpha-ketoglutarate dehydrogenase consuming CoA, overview | Escherichia coli K-12 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FabD | - |
Escherichia coli K-12 |
malonyl-CoA:acyl carrier protein transacylase | - |
Escherichia coli K-12 |
MCAT | - |
Escherichia coli K-12 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Escherichia coli K-12 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Escherichia coli K-12 |