Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a hexahistidine-tagged fusion protein | Streptomyces coelicolor |
General Stability | Organism |
---|---|
enzyme labile during chromatography | Streptomyces coelicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenylmethanesulfonyl fluoride | - |
Escherichia coli | |
phenylmethanesulfonyl fluoride | - |
Streptomyces coelicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
malonyl-CoA | hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein | Streptomyces coelicolor | |
0.073 | - |
acyl-carrier protein | hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein | Streptomyces coelicolor | |
0.25 | - |
malonyl-CoA | reaction with E. coli acyl-carrier-protein | Escherichia coli | |
0.327 | - |
malonyl-CoA | hexahistidine-tagged fusion protein, reaction with E. coli acyl-carrier-protein | Streptomyces coelicolor | |
0.351 | - |
acyl-carrier protein | reaction with E. coli acyl-carrier-protein | Escherichia coli | |
0.776 | - |
acyl-carrier protein | reaction with E. coli acyl-carrier-protein | Streptomyces coelicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34110 | - |
electrospray mass spectrometry, hexahistidine-tagged fusion protein | Streptomyces coelicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Streptomyces coelicolor | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the hexahistidine-tagged fusion protein, using Ni(II) affinity chromatography, column chromatography on Q-Sepharose, phenyl Superose and Mono-Q | Streptomyces coelicolor |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
malonyl-CoA + an [acyl-carrier protein] = CoA + a malonyl-[acyl-carrier protein] | mechanism, kinetic studies | Streptomyces coelicolor |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
additional information | - |
hexahistidine-tagged fusion protein | Streptomyces coelicolor |
780 | - |
hexahistidine-tagged fusion protein, at pH 6.5 and 30°C | Streptomyces coelicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
malonyl-CoA + acyl-carrier protein | - |
Escherichia coli | CoA + malonyl-[acyl-carrier protein] | - |
? | |
malonyl-CoA + acyl-carrier protein | - |
Streptomyces coelicolor | CoA + malonyl-[acyl-carrier protein] | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
30 | - |
assay at | Streptomyces coelicolor |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
420 | - |
malonyl CoA | hexahistidine-tagged fusion protein, reaction with E. coli acyl-carrier-protein | Streptomyces coelicolor | |
450 | - |
malonyl CoA | hexahistidine-tagged fusion protein, reaction with Streptomyces coelicolor acyl-carrier-protein | Streptomyces coelicolor | |
1580 | - |
malonyl CoA | reaction with E. coli acyl-carrier-protein | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Escherichia coli |
6.5 | - |
assay at | Streptomyces coelicolor |