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Literature summary for 2.3.1.39 extracted from
- Malonyl-CoA acyl carrier protein transacylase (1969), Methods Enzymol., 14, 53-56.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| iodoacetamide | 59% inhibition at 0.1 mM | Escherichia coli |  |
| N-ethylmaleimide | 79% inhibition at 0.1 mM | Escherichia coli | |
| sulfhydryl compounds | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Escherichia coli | 5737 | - |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using streptomycin sulfate, column chromatography on DEAE-cellulose, ammonium sulfate fractionation, chromatography on Sephadex G-100 I column, DEAE-Sephadex column and Sephadex G-100 II column | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 7.04 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 2-4 months | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malonyl-CoA + acyl-carrier protein | acetyl-CoA cannot replace malonyl-CoA | Escherichia coli | CoA + malonyl-[acyl-carrier protein] | - |
? | |
| malonyl-CoA + acyl-carrier protein | the enzyme is very specific for the malonyl group | Escherichia coli | CoA + malonyl-[acyl-carrier protein] | - |
? | |
| malonyl-CoA + pantetheine | - |
Escherichia coli | malonyl-pantetheine + CoA | - |
? | |
| malonyl-pantetheine + acyl-carrier protein | - |
Escherichia coli | pantetheine + malonyl-[acyl-carrier protein] | - |
? | |
| additional information | mercaptoethanol is inactive as a substitute for acyl-carrier-protein | Escherichia coli | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
the enzyme is quite stable to heat | Escherichia coli |
| 80 | - |
20% loss of activity after 20 min | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Escherichia coli |
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Release 2023.1 (January 2023)
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