Cloned (Comment) | Organism |
---|---|
cloning and sequencing of fadA gene, expression in Escherichia coli of wild-type and C92S mutant | Streptomyces collinus |
Protein Variants | Comment | Organism |
---|---|---|
C92S | no remaining activity acetyl-CoA:acyl carrier protein transacylase activity, but remaining strongly reduced thiolase activity | Streptomyces collinus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | acetyl-CoA protects; strong | Streptomyces collinus | |
thiolactomycin | - |
Streptomyces collinus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0043 | - |
acetyl-CoA | native wild-type enzyme | Streptomyces collinus | |
0.0087 | - |
acetyl-CoA | recombinant wild-type enzyme | Streptomyces collinus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
amino acid sequence, comparison | Streptomyces collinus |
45000 | - |
4 * 45000, SDS-PAGE | Streptomyces collinus |
185000 | - |
gel filtration | Streptomyces collinus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces collinus | - |
gene fadA | - |
Purification (Comment) | Organism |
---|---|
native wild-type and recombinant wild-type and mutant from E. coli | Streptomyces collinus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.022 | - |
purified native wild-type enzyme | Streptomyces collinus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + [acyl-carrier protein] | specific for acetyl-CoA, utilizes [acyl-carrier-protein] of diverse origins with differing affinities | Streptomyces collinus | CoA + acetyl-[acyl-carrier protein] | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 45000, SDS-PAGE | Streptomyces collinus |
Synonyms | Comment | Organism |
---|---|---|
acetyl-CoA:acyl carrier protein transacylase | - |
Streptomyces collinus |
ACT | - |
Streptomyces collinus |
More | cf. EC 2.3.1.85 and EC 2.3.1.86 | Streptomyces collinus |
More | enzyme has both a acetyl-CoA:acyl carrier protein transacylase activity and a much higher thiolase activity | Streptomyces collinus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Streptomyces collinus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces collinus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0225 | - |
acetyl-CoA | recombinant wild-type, crude Streptomyces glaucescens [acyl-carrier-protein] | Streptomyces collinus | |
0.0508 | - |
acetyl-CoA | native wild-type, crude E. coli [acyl-carrier-protein] | Streptomyces collinus |