Literature summary for 2.3.1.13 extracted from

Van der Sluis, R.; Badenhorst, C.; Van der Westhuizen, F.; Van Dijk, A.
Characterisation of the influence of genetic variations on the enzyme activity of a recombinant human glycine N-acyltransferase (2013), Gene, 515, 447-453.

Search for more literature for 2.3.1.13

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Origami cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
E227Q	inactive	Homo sapiens
F168L	the mutant shows decreased activity compared to the wild type enzyme	Homo sapiens
K16N	the mutant shows wild type activity	Homo sapiens
N156S	the mutant shows increased activity compared to the wild type enzyme	Homo sapiens
R131H	the mutant shows wild type activity	Homo sapiens
R199C	inactive	Homo sapiens
S17T	the mutant shows wild type activity	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.021	-	benzoyl-CoA	mutant enzyme K16N, apparent value, at pH 8.0 and 37°C	Homo sapiens
0.024	-	benzoyl-CoA	wild type enzyme, apparent value, at pH 8.0 and 37°C	Homo sapiens
0.028	-	benzoyl-CoA	mutant enzyme S17T, apparent value, at pH 8.0 and 37°C	Homo sapiens
0.038	-	benzoyl-CoA	mutant enzyme N156S, apparent value, at pH 8.0 and 37°C	Homo sapiens
0.053	-	benzoyl-CoA	mutant enzyme F168L, apparent value, at pH 8.0 and 37°C	Homo sapiens
0.071	-	benzoyl-CoA	mutant enzyme R131H, apparent value, at pH 8.0 and 37°C	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	x * 56000, thioredoxin-His-tagged fusion protein, SDS-PAGE	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q6IB77	-	-

Purification (Commentary)

Purification (Comment)	Organism
Protino Ni-TED 2000 column chromatography and Sephadex G25 gel filtration	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
liver	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
benzoyl-CoA + glycine	-	Homo sapiens	CoA + N-benzoylglycine	-	?

Subunits

Subunits	Comment	Organism
?	x * 56000, thioredoxin-His-tagged fusion protein, SDS-PAGE	Homo sapiens

Synonyms

Synonyms	Comment	Organism
GlyAT	-	Homo sapiens

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Release 2023.1 (January 2023)