KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
phenylacetyl-CoA | cosubstrate glycine | Oryctolagus cuniculus | |
20 | - |
glycine | acyl donor phenylacetyl-CoA | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | kidney enzyme is located in the matrix, probably not membrane bound | Rattus norvegicus | 5739 | - |
mitochondrion | kidney enzyme is located in the matrix, probably not membrane bound | Oryctolagus cuniculus | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
New Zealand white rabbits | - |
Rattus norvegicus | - |
Sprague-Dawley rats | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
kidney | - |
Oryctolagus cuniculus | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.105 | - |
activity in 176000 g supernantant of Triton X-100 solubilized preparations | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + glycine | phenylacetyl-CoA acts as acyl donor | Rattus norvegicus | CoA + N-acylglycine | - |
? | |
acyl-CoA + glycine | phenylacetyl-CoA acts as acyl donor | Oryctolagus cuniculus | CoA + N-acylglycine | - |
? | |
phenylacetyl-CoA + glycine | - |
Oryctolagus cuniculus | CoA + N-phenylacetylglycine | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | 9.5 | kidney enzyme | Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 10 | approx. 35% of maximal activity at pH 8.0, approx. 40% of maximal activity at pH 10.0, kidney enzyme | Oryctolagus cuniculus |