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Literature summary for 2.3.1.13 extracted from

  • Nandi, D.L.; Lucas, S.V.; Webster, L.T.
    Benzoyl-coenzyme A:glycine N-acyltransferase and phenylacetyl-coenzyme A:glycine N-acyltransferase from bovine liver mitochondria. Purification and characterization (1979), J. Biol. Chem., 254, 7230-7237.
    View publication on PubMed
Search for more literature for 2.3.1.13

General Stability

General Stability Organism
enzyme fractions from hydroxylapatite column are inactivated by dialysis or concentration by ultracentrifugation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
5,5'-dithiobis(2-nitrobenzoate)
-
 Bos taurus
benzoyl-CoA
-
 Bos taurus
butyryl-CoA
-
 Bos taurus
Mg2+
-
 Bos taurus
Ni2+
-
 Bos taurus
p-chloromercuribenzoate
-
 Bos taurus
Zn2+
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cs+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
K+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
Li+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
Na+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
NH4+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus
Rb+ K+, Rb+, Na+, Li+, Cs+ or NH4+ required for activity Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 SDS-PAGE, sucrose density gradient centrifugation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Reaction

Reaction Comment Organism Reaction ID
acyl-CoA + glycine = CoA + N-acylglycine sequential reaction mechanism, acyl-CoA substrate adds to the enzyme first, glycine adds before CoA leaves and the peptide product dissociates last Bos taurus
a

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Bos taurus
-

Storage Stability

Storage Stability Organism
4°C, 1-3 weeks, about 25% loss of activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + glycine phenylacetyl-CoA or indoleacetyl-CoA can not act as acyl donor Bos taurus CoA + N-acylglycine
-
 ?
acyl-CoA + L-asparagine
-
 Bos taurus ?
-
 ?
acyl-CoA + L-glutamine
-
 Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 33000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4 8.6
-
 Bos taurus