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Literature summary for 2.3.1.12 extracted from

  • Aso, Y.; Nakajima, A.; Meno, K.; Ishiguro, M.
    Thermally induced changes of lipoate acetyltransferase inner core isolated from the Bacillus stearothermophilus pyruvate dehydrogenase complex (2001), Biosci. Biotechnol. Biochem., 65, 698-701.
    View publication on PubMed
Search for more literature for 2.3.1.12

Localization

Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
dihydrolipoamide + acetyl-CoA Geobacillus stearothermophilus
-
 S-acetyldihydrolipoamide + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Geobacillus stearothermophilus

Renatured (Commentary)

Renatured (Comment) Organism
treatment with guanidine hydrochloride and its subsequent removal results in little recovery of the core, but full recovery of X Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dihydrolipoamide + acetyl-CoA
-
 Geobacillus stearothermophilus S-acetyldihydrolipoamide + CoA
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 incubation converts core of the enzyme into an unidentified active molecular form X, loss of 10% enzyme activity after 5 h Geobacillus stearothermophilus
78
-
 temperatures below result in less than 5% loss in enzyme activity Geobacillus stearothermophilus
80
-
 partly precipitation above Geobacillus stearothermophilus