General Stability | Organism |
---|---|
freezing and thawing does not cause loss of activity | Sus scrofa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
SDS-PAGE and sedimentation equilibrium data | Sus scrofa |
1800000 | 1970000 | calculation from sedimentation and diffusion constants, meniscus depletion method | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + acetyl-CoA | Sus scrofa | - |
S-acetyldihydrolipoamide + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
lipoprotein | 23 mol of lipoic acid per mol of enzyme | Sus scrofa |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Renatured (Comment) | Organism |
---|---|
reconstitution of pyruvate dehydrogenase complex, optimum catalytic stoichiometry of E1:E2:E3 is 5.2:11.5:2 | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
19.7 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-18°C, 0.05 mM potassium phosphate buffer, pH 7.0, 0.5 mM EDTA, more than 6 months | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dihydrolipoamide + acetyl-CoA | - |
Sus scrofa | S-acetyldihydrolipoamide + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
polymer | 24 * 74000 SDS-PAGE, gel filtration in 6 M guanidine-HCl, sedimentation equilibrium in 6 M guanidine-HCl | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
- |
Sus scrofa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable up to | Sus scrofa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | 8.5 | - |
Sus scrofa |