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Literature summary for 2.3.1.108 extracted from

  • Davenport, A.M.; Collins, L.N.; Chiu, H.; Minor, P.J.; Sternberg, P.W.; Hoelz, A.
    Structural and functional characterization of the alpha-tubulin acetyltransferase MEC-17 (2014), J. Mol. Biol., 426, 2605-2616.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.108

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure of the catalytic core of human MEC-17 in complex with its cofactor acetyl-CoA at 1.7 A resolution. The MEC-17 core adopts a canonical Gcn5-related N-acetyltransferase (GNAT) fold that is decorated with extensive surface loops. A large, evolutionarily conserved hydrophobic surface patch is critical for enzymatic activity Homo sapiens

Protein Variants

Protein Variants Comment Organism
C120A complete loss of activity Homo sapiens
D157A complete loss of activity Homo sapiens
D157N complete loss of activity Homo sapiens
F105A complete loss of activity Homo sapiens
F105A mutation in highly conserved surface patches adjacent to the substrate-binding groove, pronounced effetc on catalytic activity Homo sapiens
F183A complete loss of activity Homo sapiens
F183A mutation in highly conserved surface patches adjacent to the substrate-binding groove, pronounced effetc on catalytic activity Homo sapiens
F186A mutation in highly conserved surface patches adjacent to the substrate-binding groove, pronounced effetc on catalytic activity Homo sapiens
F190A mutation in highly conserved surface patches adjacent to the substrate-binding groove, pronounced effetc on catalytic activity Homo sapiens
I64A complete loss of activity Homo sapiens
K102A complete loss of activity Homo sapiens
K103A about 30% of wild-type activity Homo sapiens
K162A mutation in the acetyl-CoA binding pocket, mild effect on enzymatic activity Homo sapiens
K169A mutation in the acetyl-CoA binding pocket, mild effect on enzymatic activity Homo sapiens
K98A complete loss of activity Homo sapiens
L104A complete loss of activity Homo sapiens
L104A mutation in highly conserved surface patches adjacent to the substrate-binding groove, pronounced effetc on catalytic activity Homo sapiens
L122A complete loss of activity Homo sapiens
L164A mutation in highly conserved surface patches adjacent to the substrate-binding groove, pronounced effetc on catalytic activity Homo sapiens
L173A mutation in highly conserved surface patches adjacent to the substrate-binding groove, pronounced effetc on catalytic activity Homo sapiens
L60A complete loss of activity Homo sapiens
additional information expression of truncated variants, residues 1-193 and residues 1-236. Truncation mutant 1-193 exhibits a 3 times higher Km value than 1-236 Homo sapiens
N181A complete loss of activity Homo sapiens
N182A about 25% of wild-type activity Homo sapiens
N73A mutation in highly conserved surface patches adjacent to the substrate-binding groove, discernable effect on catalytic activity Homo sapiens
P159A about 10% of wild-type activity Homo sapiens
P178A mutation in highly conserved surface patches adjacent to the substrate-binding groove, pronounced effetc on catalytic activity Homo sapiens
Q131A mutation in the acetyl-CoA binding pocket, mild effect on enzymatic activity Homo sapiens
Q179A complete loss of activity Homo sapiens
Q179A mutation in highly conserved surface patches adjacent to the substrate-binding groove, pronounced effetc on catalytic activity Homo sapiens
Q58A complete loss of activity Homo sapiens
R132A mutation in the acetyl-CoA binding pocket, mild effect on enzymatic activity Homo sapiens
R158A about 45% of wild-type activity Homo sapiens
R69A about 10% of wild-type activity Homo sapiens
S160A mutation in the acetyl-CoA binding pocket, mild effect on enzymatic activity Homo sapiens
S66A mutation in highly conserved surface patches adjacent to the substrate-binding groove, discernable effect on catalytic activity Homo sapiens
V184A mutation in highly conserved surface patches adjacent to the substrate-binding groove, pronounced effetc on catalytic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0035
-
 alpha-tubulin L-lysine mutant L164A, pH 6.9, 22°C Homo sapiens
0.0136
-
 alpha-tubulin L-lysine truncation variant 1-236, pH 6.9, 22°C Homo sapiens
0.0146
-
 alpha-tubulin L-lysine mutant Q131A, pH 6.9, 22°C Homo sapiens
0.0152
-
 alpha-tubulin L-lysine mutant F186A, pH 6.9, 22°C Homo sapiens
0.0188
-
 alpha-tubulin L-lysine mutant P178A, pH 6.9, 22°C Homo sapiens
0.0234
-
 alpha-tubulin L-lysine mutant N73A, pH 6.9, 22°C Homo sapiens
0.0312
-
 alpha-tubulin L-lysine mutant K103A, pH 6.9, 22°C Homo sapiens
0.0368
-
 alpha-tubulin L-lysine mutant L173A, pH 6.9, 22°C Homo sapiens
0.0369
-
 alpha-tubulin L-lysine mutant P159A, pH 6.9, 22°C Homo sapiens
0.0433
-
 alpha-tubulin L-lysine mutant K169A, pH 6.9, 22°C Homo sapiens
0.0454
-
 alpha-tubulin L-lysine truncation variant 1-193, pH 6.9, 22°C Homo sapiens
0.0471
-
 alpha-tubulin L-lysine mutant V184A, pH 6.9, 22°C Homo sapiens
0.0484
-
 alpha-tubulin L-lysine mutant K162A, pH 6.9, 22°C Homo sapiens
0.0509
-
 alpha-tubulin L-lysine mutant S160A, pH 6.9, 22°C Homo sapiens
0.052
-
 alpha-tubulin L-lysine mutant R132A, pH 6.9, 22°C Homo sapiens
0.0582
-
 alpha-tubulin L-lysine mutant N182A, pH 6.9, 22°C Homo sapiens
0.0795
-
 alpha-tubulin L-lysine mutant R158A, pH 6.9, 22°C Homo sapiens
0.0843
-
 alpha-tubulin L-lysine mutant S66A, pH 6.9, 22°C Homo sapiens
0.0984
-
 alpha-tubulin L-lysine mutant R69A, pH 6.9, 22°C Homo sapiens
0.1073
-
 alpha-tubulin L-lysine mutant F190A, pH 6.9, 22°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q5SQI0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + alpha-tubulin L-lysine
-
 Homo sapiens CoA + alpha-tubulin N6-acetyl-L-lysine
-
 ?

Synonyms

Synonyms Comment Organism
ATAT1
-
 Homo sapiens
Mec-17
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
 alpha-tubulin L-lysine mutant L164A, pH 6.9, 22°C Homo sapiens
0.8
-
 alpha-tubulin L-lysine mutant F186A, pH 6.9, 22°C Homo sapiens
1
-
 alpha-tubulin L-lysine mutant P159A, pH 6.9, 22°C Homo sapiens
2
-
 alpha-tubulin L-lysine mutant L173A, pH 6.9, 22°C Homo sapiens
2.4
-
 alpha-tubulin L-lysine mutant P178A, pH 6.9, 22°C Homo sapiens
2.8
-
 alpha-tubulin L-lysine mutant K103A, pH 6.9, 22°C Homo sapiens
2.8
-
 alpha-tubulin L-lysine mutant Q131A, pH 6.9, 22°C Homo sapiens
2.8
-
 alpha-tubulin L-lysine mutant R69A, pH 6.9, 22°C Homo sapiens
3.2
-
 alpha-tubulin L-lysine truncation variant 1-193, pH 6.9, 22°C Homo sapiens
3.3
-
 alpha-tubulin L-lysine mutant R132A, pH 6.9, 22°C Homo sapiens
3.5
-
 alpha-tubulin L-lysine mutant K169A, pH 6.9, 22°C Homo sapiens
3.7
-
 alpha-tubulin L-lysine mutant S160A, pH 6.9, 22°C Homo sapiens
3.8
-
 alpha-tubulin L-lysine truncation variant 1-236, pH 6.9, 22°C Homo sapiens
4.1
-
 alpha-tubulin L-lysine mutant N182A, pH 6.9, 22°C Homo sapiens
4.8
-
 alpha-tubulin L-lysine mutant R158A, pH 6.9, 22°C Homo sapiens
4.9
-
 alpha-tubulin L-lysine mutant N73A, pH 6.9, 22°C Homo sapiens
5.8
-
 alpha-tubulin L-lysine mutant V184A, pH 6.9, 22°C Homo sapiens
6
-
 alpha-tubulin L-lysine mutant F190A, pH 6.9, 22°C Homo sapiens
6.5
-
 alpha-tubulin L-lysine mutant K162A, pH 6.9, 22°C Homo sapiens
9.2
-
 alpha-tubulin L-lysine mutant S66A, pH 6.9, 22°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
260
-
 alpha-tubulin L-lysine mutant P159A, pH 6.9, 22°C Homo sapiens
280
-
 alpha-tubulin L-lysine mutant R69A, pH 6.9, 22°C Homo sapiens
510
-
 alpha-tubulin L-lysine mutant F186A, pH 6.9, 22°C Homo sapiens
540
-
 alpha-tubulin L-lysine mutant L173A, pH 6.9, 22°C Homo sapiens
560
-
 alpha-tubulin L-lysine mutant F190A, pH 6.9, 22°C Homo sapiens
610
-
 alpha-tubulin L-lysine mutant R158A, pH 6.9, 22°C Homo sapiens
630
-
 alpha-tubulin L-lysine mutant R132A, pH 6.9, 22°C Homo sapiens
710
-
 alpha-tubulin L-lysine mutant N182A, pH 6.9, 22°C Homo sapiens
710
-
 alpha-tubulin L-lysine truncation variant 1-193, pH 6.9, 22°C Homo sapiens
720
-
 alpha-tubulin L-lysine mutant S160A, pH 6.9, 22°C Homo sapiens
800
-
 alpha-tubulin L-lysine mutant K169A, pH 6.9, 22°C Homo sapiens
890
-
 alpha-tubulin L-lysine mutant K103A, pH 6.9, 22°C Homo sapiens
1100
-
 alpha-tubulin L-lysine mutant S66A, pH 6.9, 22°C Homo sapiens
1220
-
 alpha-tubulin L-lysine mutant V184A, pH 6.9, 22°C Homo sapiens
1270
-
 alpha-tubulin L-lysine mutant P178A, pH 6.9, 22°C Homo sapiens
1340
-
 alpha-tubulin L-lysine mutant K162A, pH 6.9, 22°C Homo sapiens
1620
-
 alpha-tubulin L-lysine mutant L164A, pH 6.9, 22°C Homo sapiens
1880
-
 alpha-tubulin L-lysine mutant Q131A, pH 6.9, 22°C Homo sapiens
2100
-
 alpha-tubulin L-lysine mutant N73A, pH 6.9, 22°C Homo sapiens
2780
-
 alpha-tubulin L-lysine truncation variant 1-236, pH 6.9, 22°C Homo sapiens