Cloned (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sulfometuron methyl | - |
Geobacillus stearothermophilus | |
valine | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.8 | - |
pyruvate | holoenzyme, pH 8.0, 60°C | Geobacillus stearothermophilus | |
12 | - |
pyruvate | large subunit, pH 8.0, 60°C | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | K0.5 value for holoenzyme 0.02 mM, for the isolated large subunit 1.3 mM | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
18700 | - |
x * 63300, large subunit IlvB, plus x * 18700, small subunit IlvN, SDS-PAGE and calculated. | Geobacillus stearothermophilus |
63300 | - |
x * 63300, large subunit IlvB, plus x * 18700, small subunit IlvN, SDS-PAGE and calculated. | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q8RL88 and Q8RL87 | Q8RL88 i.e. large subunit IlvB, Q8RL87 i.e. small subunit IlvN | - |
Renatured (Comment) | Organism |
---|---|
the isolated large subunit has about one-third the activity of the holoenzyme, but it can be reconstituted to nearly complete activity by the addition of purified small subunits | Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.12 | - |
large subunit, pH 7.9, 55°C | Geobacillus stearothermophilus |
9.16 | - |
holoenzyme, pH 7.9, 55°C | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Geobacillus stearothermophilus | (S)-acetolactate + CO2 | - |
? | |
2-oxobutanoate + pyruvate | - |
Geobacillus stearothermophilus | 2-hydroxy-2-methyl-3-oxopentanoate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 63300, large subunit IlvB, plus x * 18700, small subunit IlvN, SDS-PAGE and calculated. | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
hlaf-life 5 min | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
holoenzyme | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Geobacillus stearothermophilus | |
thiamine diphosphate | K0.5 value for holoenzyme 0.0055 mM, for the isolated large subunit 0.24 mM | Geobacillus stearothermophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
sulfometuron methyl | holoenzyme, pH 7.9, 55°C | Geobacillus stearothermophilus | |
0.018 | - |
valine | holoenzyme, pH 8.0, 60°C | Geobacillus stearothermophilus |