Cloned (Comment) | Organism |
---|---|
gene ahas1 encoding the AHAS catalytic subunit, DNA and amino acid sequence determination and analysis, expression pattern of ahas genes family in sunflower | Helianthus annuus |
gene ahas2 encoding the AHAS catalytic subunit, DNA and amino acid sequence determination and analysis, expression pattern of ahas genes family in sunflower | Helianthus annuus |
gene ahas3 encoding the AHAS catalytic subunit, DNA and amino acid sequence determination and analysis, expression pattern of ahas genes family in sunflower | Helianthus annuus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Helianthus annuus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | Helianthus annuus | - |
2-acetolactate + CO2 | - |
? | |
2 pyruvate | Helianthus annuus HA89 | - |
2-acetolactate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | Helianthus annuus | - |
2-aceto-2-hydroxybutyrate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | Helianthus annuus HA89 | - |
2-aceto-2-hydroxybutyrate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus annuus | - |
inbred line HA89 | - |
Helianthus annuus | Q5VB42 | isozyme AHAS3; gene ahas3 encoding the AHAS catalytic subunit | - |
Helianthus annuus | Q5VB44 | isozyme AHAS2; gene ahas2 encoding the AHAS catalytic subunit | - |
Helianthus annuus | Q5VB49 | isozyme AHAS1; gene ahas1 encoding the AHAS catalytic subunit | - |
Helianthus annuus HA89 | Q5VB42 | isozyme AHAS3; gene ahas3 encoding the AHAS catalytic subunit | - |
Helianthus annuus HA89 | Q5VB44 | isozyme AHAS2; gene ahas2 encoding the AHAS catalytic subunit | - |
Helianthus annuus HA89 | Q5VB49 | isozyme AHAS1; gene ahas1 encoding the AHAS catalytic subunit | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
embryo | - |
Helianthus annuus | - |
embryo | maximal expression of ahas2 in immature flowers and embryos | Helianthus annuus | - |
embryo | maximal expression of ahas3 in immature flowers and embryos | Helianthus annuus | - |
embryo | maximal expression of isoforms ahas2 and ahas3 in embryo | Helianthus annuus | - |
flower | - |
Helianthus annuus | - |
flower | maximal expression of ahas2 in immature flowers and embryos | Helianthus annuus | - |
flower | maximal expression of ahas3 in immature flowers and embryos | Helianthus annuus | - |
flower | maximal expression of isoforms ahas2 and ahas3 in immature flowers | Helianthus annuus | - |
hypocotyl | - |
Helianthus annuus | - |
leaf | - |
Helianthus annuus | - |
leaf | highest expression for gene ahas1 in leaves | Helianthus annuus | - |
leaf | highest expression and activity of isoform ahas1 | Helianthus annuus | - |
additional information | the conservation of multiple AHAS isoforms in sunflower seems to result from different expression requirements controlled by tissue-specific regulatory mechanisms at different developmental stages, tissue-specific localization and activity of AHAS isozymes evaluated at several developmental stages, transcriptional levels of ahas genes, overview | Helianthus annuus | - |
root | - |
Helianthus annuus | - |
seedling | - |
Helianthus annuus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Helianthus annuus | 2-acetolactate + CO2 | - |
? | |
2 pyruvate | - |
Helianthus annuus HA89 | 2-acetolactate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | - |
Helianthus annuus | 2-aceto-2-hydroxybutyrate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | - |
Helianthus annuus HA89 | 2-aceto-2-hydroxybutyrate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetohydroxyacid synthase | - |
Helianthus annuus |
AHAS | - |
Helianthus annuus |
AHAS1 | - |
Helianthus annuus |
AHAS2 | - |
Helianthus annuus |
AHAS3 | - |
Helianthus annuus |
ALS | - |
Helianthus annuus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Helianthus annuus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Helianthus annuus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Helianthus annuus | |
thiamine diphosphate | dependent on | Helianthus annuus |
General Information | Comment | Organism |
---|---|---|
metabolism | enzyme AHAS catalyses the condensation of either two molecules of pyruvate to form acetolactate in the leucine and valine pathway, or of one molecule of pyruvate with one molecule of 2-oxobutyrate toform 2-aceto-2-hydroxybutyrate as the first step in the isoleucine biosynthesis | Helianthus annuus |