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Literature summary for 2.2.1.6 extracted from

  • Guo, X.W.; Zhang, Y.H.; Cao, C.H.; Shen, T.; Wu, M.Y.; Chen, Y.F.; Zhang, C.Y.; Xiao, D.G.
    Enhanced production of 2,3-butanediol by overexpressing acetolactate synthase and acetoin reductase in Klebsiella pneumoniae (2014), Biotechnol. Appl. Biochem., 61, 707-715.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Klebsiella pneumoniae KG-rs cells Klebsiella pneumoniae
gene als, cloning in Escherichia coli strain DH5alpha Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information mutants with overexpression of 2-acetolactate synthase (ALS), 2-acetolactate decarboxylase, and acetoin reductase, either individually or in combination, are constructed to improve 2,3-butanediol production in Klebsiella pneumoniae. The strain (KG-rs) that overexpresses both 2-acetolactate synthase and acetoin reductase shows an improved 2,3-butanediol yield. When cultured in the media with five different carbon sources (glucose, galactose, fructose, sucrose, and lactose), the mutant exhibits higher 2,3-butanediol productivity and production than the parental strain in all the tested carbon sources except for lactose. The 2,3-butanediol production of strain KG-rs in a batch fermentation with glucose as the carbon source is 12% higher than that of the parental strain, overview Klebsiella pneumoniae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
x * 60000, SDS-PAGE Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 pyruvate Klebsiella pneumoniae
-
2-acetolactate + CO2
-
?
2 pyruvate Klebsiella pneumoniae KG1
-
2-acetolactate + CO2
-
?
2 pyruvate Klebsiella pneumoniae KG-1
-
2-acetolactate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
-
-
Klebsiella pneumoniae KG-1
-
-
-
Klebsiella pneumoniae KG1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
Klebsiella pneumoniae 2-acetolactate + CO2
-
?
2 pyruvate
-
Klebsiella pneumoniae KG1 2-acetolactate + CO2
-
?
2 pyruvate
-
Klebsiella pneumoniae KG-1 2-acetolactate + CO2
-
?
additional information detection of nonenzymatic acetoin formation from acetolactate in the assay Klebsiella pneumoniae ?
-
?
additional information detection of nonenzymatic acetoin formation from acetolactate in the assay Klebsiella pneumoniae KG1 ?
-
?
additional information detection of nonenzymatic acetoin formation from acetolactate in the assay Klebsiella pneumoniae KG-1 ?
-
?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Klebsiella pneumoniae

Synonyms

Synonyms Comment Organism
alpha-acetolactate synthase
-
Klebsiella pneumoniae
ALS
-
Klebsiella pneumoniae

General Information

General Information Comment Organism
metabolism fermentation pathways in Klebsiella pneumoniae, overview. The biosynthesis route of 2,3-BD in Klebsiella pneumoniae proceeds via pyruvate, acetolactate, and acetoin to 2,3-BD. 2,3-BD production from pyruvate involves three enzymes, namely, 2-acetolactate synthase (ALS), 2-acetolactate decarboxylase (ALDC), and acetoin reductase (AR). These enzymes catalyze the production of acetolactate from pyruvate, acetoin from acetolactate, and 2,3-BD from acetoin Klebsiella pneumoniae