Protein Variants | Comment | Organism |
---|---|---|
C83A | about 91% of wild-type activity | Escherichia coli |
C83S | about 126% of wild-type activity | Escherichia coli |
C83T | about 41% of wild-type activity | Escherichia coli |
E60A | about 48% of wild-type activity | Escherichia coli |
E60Q | about 1% of wild-type activity | Escherichia coli |
L476M | about 34% of wild-type activity | Escherichia coli |
L476M/Q480W | about 47% of wild-type activity | Escherichia coli |
M263A | about 16% of wild-type activity | Escherichia coli |
Q480W | about 22% of wild-type activity | Escherichia coli |
R269Q | about 0.5% of wild-type activity | Escherichia coli |
R289K | about 11% of wild-type activity | Escherichia coli |
V391A | about 3% of wild-type activity | Escherichia coli |
V477I | about 8% of wild-type activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sulfometuron methyl | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
pyruvate | mutant C83T, pH 7.6, 37°C | Escherichia coli | |
1.2 | - |
pyruvate | mutant C83S, pH 7.6, 37°C | Escherichia coli | |
1.5 | - |
pyruvate | mutant C83A, pH 7.6, 37°C | Escherichia coli | |
3.6 | - |
pyruvate | mutant L476M/Q480W, pH 7.6, 37°C | Escherichia coli | |
4.7 | - |
pyruvate | mutant Q480W, pH 7.6, 37°C | Escherichia coli | |
4.8 | - |
pyruvate | wild-type, pH 7.6, 37°C | Escherichia coli | |
8 | - |
pyruvate | mutant V477I, pH 7.6, 37°C | Escherichia coli | |
13 | - |
pyruvate | mutant L476M, pH 7.6, 37°C | Escherichia coli | |
17.9 | - |
pyruvate | mutant V391A, pH 7.6, 37°C | Escherichia coli | |
21 | - |
pyruvate | mutant E60Q, pH 7.6, 37°C | Escherichia coli | |
26 | - |
pyruvate | mutant M263A, pH 7.6, 37°C | Escherichia coli | |
28 | - |
pyruvate | mutant E60A, pH 7.6, 37°C | Escherichia coli | |
50 | - |
pyruvate | mutant R289K, pH 7.6, 37°C | Escherichia coli | |
124 | - |
pyruvate | mutant R289Q, pH 7.6, 37°C | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isozyme I | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 pyruvate | - |
Escherichia coli | 2-acetolactate + CO2 | - |
? | |
2-oxobutanoate + pyruvate | - |
Escherichia coli | 2-hydroxy-2-methyl-3-oxopentanoate + CO2 | - |
? | |
additional information | isozyme I is not specific for 2-oxobutanoate over pyruvate as an acceptor substrate. Residues Gln480 and Met476 in AHAS I replace the Trp and Leu residues conserved in other acetohydroxyacid synthases and lead to accelerated ligation and product release steps. This difference in kinetics accounts for the unique specificity, reversibility and allosteric response of AHAS I | Escherichia coli | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
pyruvate | mutant C83T, pH 7.6, 37°C | Escherichia coli | |
2 | - |
pyruvate | mutant E60A, pH 7.6, 37°C | Escherichia coli | |
2 | - |
pyruvate | mutant E60Q, pH 7.6, 37°C | Escherichia coli | |
6 | - |
pyruvate | mutant R289Q, pH 7.6, 37°C | Escherichia coli | |
8 | - |
pyruvate | mutant V391A, pH 7.6, 37°C | Escherichia coli | |
9 | - |
pyruvate | mutant V477I, pH 7.6, 37°C | Escherichia coli | |
15 | - |
pyruvate | mutant Q480W, pH 7.6, 37°C | Escherichia coli | |
20 | - |
pyruvate | mutant C83A, pH 7.6, 37°C | Escherichia coli | |
23 | - |
pyruvate | mutant C83S, pH 7.6, 37°C | Escherichia coli | |
25 | - |
pyruvate | mutant L476M/Q480W, pH 7.6, 37°C | Escherichia coli | |
62 | - |
pyruvate | mutant M263A, pH 7.6, 37°C | Escherichia coli | |
65 | - |
pyruvate | mutant L476M, pH 7.6, 37°C | Escherichia coli | |
71 | - |
pyruvate | wild-type, pH 7.6, 37°C | Escherichia coli | |
86 | - |
pyruvate | mutant R289K, pH 7.6, 37°C | Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0044 | - |
sulfometuron methyl | mutant Q480W, pH 7.6, 37°C | Escherichia coli | |
0.041 | - |
sulfometuron methyl | mutant L476M/Q480W, pH 7.6, 37°C | Escherichia coli |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
pyruvate | mutant R289Q, pH 7.6, 37°C | Escherichia coli | |
0.07 | - |
pyruvate | mutant E60A, pH 7.6, 37°C | Escherichia coli | |
0.1 | - |
pyruvate | mutant E60Q, pH 7.6, 37°C | Escherichia coli | |
0.46 | - |
pyruvate | mutant V391A, pH 7.6, 37°C | Escherichia coli | |
1.2 | - |
pyruvate | mutant V477I, pH 7.6, 37°C | Escherichia coli | |
1.7 | - |
pyruvate | mutant R289K, pH 7.6, 37°C | Escherichia coli | |
2.4 | - |
pyruvate | mutant M263A, pH 7.6, 37°C | Escherichia coli | |
3.3 | - |
pyruvate | mutant Q480W, pH 7.6, 37°C | Escherichia coli | |
5 | - |
pyruvate | mutant L476M, pH 7.6, 37°C | Escherichia coli | |
6.1 | - |
pyruvate | mutant C83T, pH 7.6, 37°C | Escherichia coli | |
6.9 | - |
pyruvate | mutant L476M/Q480W, pH 7.6, 37°C | Escherichia coli | |
13.5 | - |
pyruvate | mutant C83A, pH 7.6, 37°C | Escherichia coli | |
14.9 | - |
pyruvate | wild-type, pH 7.6, 37°C | Escherichia coli | |
18.8 | - |
pyruvate | mutant C83S, pH 7.6, 37°C | Escherichia coli |