Application | Comment | Organism |
---|---|---|
drug development | AHAS is the target of the sulfonylurea and imidazolinone herbicides | Escherichia coli |
drug development | AHAS is the target of the sulfonylurea and imidazolinone herbicides | Saccharomyces cerevisiae |
Crystallization (Comment) | Organism |
---|---|
crystal structure analysis | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
additional information | isozyme AHAS II mutants of residues Phe109, Met250, Arg276 and Trp464 are nearly inactive in (S)-2-acetolactate formation, but show increased activity with pyruvate and benzaldehyde compared to the wild-type isozyme | Escherichia coli |
W464L | the mutant of isozyme AHAS II has lost the preference for 2-ketobutyrate as second substrate | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
branched-chain amino acids | feedback inhibition, differential inhibition of isozymes, overview | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Escherichia coli | the enzyme is the first common enzyme in the pathway for the biosynthesis of branched-chain amino acids, overview | (S)-2-acetolactate + CO2 | - |
? | |
pyruvate | Saccharomyces cerevisiae | the enzyme is the first common enzyme in the pathway for the biosynthesis of branched-chain amino acids, overview | (S)-2-acetolactate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | Escherichia coli | stereospecific reaction | (S)-acetohydroxybutyrate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
isozymes I-III | - |
Saccharomyces cerevisiae | P07342 | - |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
2 pyruvate = 2-acetolactate + CO2 | catalytic mechanism and active site structure, covalent intermediates | Saccharomyces cerevisiae | |
2 pyruvate = 2-acetolactate + CO2 | catalytic mechanism, covalent intermediates | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | substrate specificity ratios of isozymes I-III, substrate recognition mechanism, overview | Escherichia coli | ? | - |
? | |
pyruvate | stereospecific reaction | Escherichia coli | (S)-2-acetolactate + CO2 | - |
? | |
pyruvate | stereospecific reaction | Saccharomyces cerevisiae | (S)-2-acetolactate + CO2 | - |
? | |
pyruvate | the enzyme is the first common enzyme in the pathway for the biosynthesis of branched-chain amino acids, overview | Escherichia coli | (S)-2-acetolactate + CO2 | - |
? | |
pyruvate | the enzyme is the first common enzyme in the pathway for the biosynthesis of branched-chain amino acids, overview | Saccharomyces cerevisiae | (S)-2-acetolactate + CO2 | - |
? | |
pyruvate + 2-oxobutyrate | stereospecific reaction | Escherichia coli | (S)-acetohydroxybutyrate + CO2 | - |
? | |
pyruvate + benzaldehyde | stereospecific reaction, benzaldehyde is an artificial substrate, especially of mutants of isozyme AHAS II residues Phe109, Met250, Arg276 and Trp464 | Escherichia coli | (R)-phenylacetylcarbinol + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
acetohydroxy acid synthase | - |
Escherichia coli |
acetohydroxy acid synthase | - |
Saccharomyces cerevisiae |
AHAS | - |
Escherichia coli |
AHAS | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Escherichia coli | |
FAD | - |
Saccharomyces cerevisiae | |
thiamine diphosphate | - |
Escherichia coli | |
thiamine diphosphate | - |
Saccharomyces cerevisiae |