Literature summary for 2.2.1.6 extracted from

Elisakova, V.; Patek, M.; Holatko, J.; Nesvera, J.; Leyval, D.; Goergen, J.L.; Delaunay, S.
Feedback-resistant acetohydroxy acid synthase increases valine production in Corynebacterium glutamicum (2005), Appl. Environ. Microbiol., 71, 207-213.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression of wild-type and mutant enzymes via shuttle vector pECKA in Escherichia coli and in Corynebacterium glutamicum	Corynebacterium glutamicum

Protein Variants

Protein Variants	Comment	Organism
additional information	mutations of the residues M8 and M13 of the small, regulatory subunit encoded by gene ilvN result in reduced sensitivity of the mutant enzymes to feedback inhibition which leads to increased production of valine as well as of isoleucine and leucine	Corynebacterium glutamicum

Inhibitors

Inhibitors	Comment	Organism	Structure
isoleucine	feedback inhibition of wild-type enzyme about 50% at 10 mM, M8 and M13 mutants are resistant	Corynebacterium glutamicum
leucine	feedback inhibition of wild-type enzyme about 50% at 10 mM, M8 and M13 mutants are resistant	Corynebacterium glutamicum
valine	feedback inhibition of wild-type enzyme about 50% at 10 mM, M8 and M13 mutants are resistant	Corynebacterium glutamicum

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
pyruvate	Corynebacterium glutamicum	the enzyme catalyzes the first committed step in the biosynthesis of valine, leucine, and isoleucine, overview	2-acetolactate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Corynebacterium glutamicum	-	genes ilvBN of the ilvBNC operon, genes ilvB and ilvN encode the catalytic and the small, regulatory subunits, respectively	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
pyruvate	-	Corynebacterium glutamicum	2-acetolactate + CO2	-	?
pyruvate	the enzyme catalyzes the first committed step in the biosynthesis of valine, leucine, and isoleucine, overview	Corynebacterium glutamicum	2-acetolactate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
acetohydroxyacid synthase	-	Corynebacterium glutamicum
AHAS	-	Corynebacterium glutamicum

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.3	-	assay at	Corynebacterium glutamicum

Cofactor

Cofactor	Comment	Organism	Structure
FAD	-	Corynebacterium glutamicum
thiamine diphosphate	-	Corynebacterium glutamicum

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Release 2023.1 (January 2023)