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Literature summary for 2.2.1.6 extracted from

  • Kim, J.; Beak, D.G.; Kim, Y.T.; Choi, J.D.; Yoon, M.Y.
    Effects of deletions at the C-terminus of tobacco acetohydroxyacid synthase on the enzyme activity and cofactor binding (2004), Biochem. J., 384, 59-68.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
DELTA567 deletion of entire C-terminus including mobile loop and C-terminal lid, no enzymic activity Nicotiana tabacum
DELTA567-582 deletion of mobile loop region 4.5% of activity compared to wild-type, increase in activation constant of thiamine diphosphate Nicotiana tabacum
DELTA598 deletion of c-terminus maintaining mobile loop and C-terminal lid, 1.2% of activity compared to wild-type Nicotiana tabacum
DELTA630 deletion of C-terminal lid, 4.5% of activity compared to wild-type, increase in activation constant of thiamine diphosphate Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.063
-
mutant DELTA567-582, 37°C, pH 7.4 Nicotiana tabacum
0.068
-
mutant DELTA630, 37°C, pH 7.4 Nicotiana tabacum
1.73
-
wild-type, 37°C, pH 7.4 Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
Nicotiana tabacum 2-acetolactate + CO2
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.028
-
pyruvate mutant DELTA598, 37°C, pH 7.4 Nicotiana tabacum
0.097
-
pyruvate mutant DELTA567-582, 37°C, pH 7.4 Nicotiana tabacum
0.104
-
pyruvate mutant DELTA630, 37°C, pH 7.4 Nicotiana tabacum
2.32
-
pyruvate wild-type, 37°C, pH 7.4 Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Nicotiana tabacum