Protein Variants | Comment | Organism |
---|---|---|
DELTA567 | deletion of entire C-terminus including mobile loop and C-terminal lid, no enzymic activity | Nicotiana tabacum |
DELTA567-582 | deletion of mobile loop region 4.5% of activity compared to wild-type, increase in activation constant of thiamine diphosphate | Nicotiana tabacum |
DELTA598 | deletion of c-terminus maintaining mobile loop and C-terminal lid, 1.2% of activity compared to wild-type | Nicotiana tabacum |
DELTA630 | deletion of C-terminal lid, 4.5% of activity compared to wild-type, increase in activation constant of thiamine diphosphate | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.063 | - |
mutant DELTA567-582, 37°C, pH 7.4 | Nicotiana tabacum |
0.068 | - |
mutant DELTA630, 37°C, pH 7.4 | Nicotiana tabacum |
1.73 | - |
wild-type, 37°C, pH 7.4 | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Nicotiana tabacum | 2-acetolactate + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
pyruvate | mutant DELTA598, 37°C, pH 7.4 | Nicotiana tabacum | |
0.097 | - |
pyruvate | mutant DELTA567-582, 37°C, pH 7.4 | Nicotiana tabacum | |
0.104 | - |
pyruvate | mutant DELTA630, 37°C, pH 7.4 | Nicotiana tabacum | |
2.32 | - |
pyruvate | wild-type, 37°C, pH 7.4 | Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Nicotiana tabacum |