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Literature summary for 2.2.1.6 extracted from

  • Yoon, T.Y.; Chung, S.M.; Chang, S.I.; Yoon, M.Y.; Hahn, T.R.; Choi, J.D.
    Roles of lysine 219 and 255 residues in tobacco acetolactate synthase (2002), Biochem. Biophys. Res. Commun., 293, 433-439.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
K219Q no residual activity, no binding of FAD Nicotiana tabacum
K255F strong resistance to Londax, Cadre and N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide Nicotiana tabacum
K255Q strong resistance to Londax, Cadre and N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide Nicotiana tabacum
K299Q no significant effects Nicotiana tabacum

Inhibitors

Inhibitors Comment Organism Structure
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one
-
Nicotiana tabacum
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide
-
Nicotiana tabacum
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide ratio 1:1 Nicotiana tabacum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
17.06
-
pyruvate wild-type, pH 7.5, 37°C Nicotiana tabacum
26.23
-
pyruvate mutant K299Q pH 7.5, 37°C Nicotiana tabacum
109.4
-
pyruvate mutant K255F, pH 7.5, 37°C Nicotiana tabacum
115.5
-
pyruvate mutant K255Q, pH 7.5, 37°C Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.02
-
mutant K255F, pH 7.5, 37°C Nicotiana tabacum
1.87
-
wild-type, pH 7.5, 37°C Nicotiana tabacum
2.17
-
mutant K255Q, pH 7.5, 37°C Nicotiana tabacum
4.15
-
mutant K299Q pH 7.5, 37°C Nicotiana tabacum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
Nicotiana tabacum 2-acetolactate + CO2
-
?

Cofactor

Cofactor Comment Organism Structure
FAD
-
Nicotiana tabacum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00000823
-
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide wild-type, pH 7.5, 37°C Nicotiana tabacum
0.00000902
-
N-[[(4-methoxy-1,3,5-triazin-2-yl)amino]carbonyl]-1-phenylmethanesulfonamide - methyl hydroperoxide mutant K299Q pH 7.5, 37°C Nicotiana tabacum
0.00109
-
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one mutant K299Q pH 7.5, 37°C Nicotiana tabacum
0.00122
-
2-(5-ethyl-3-methylpyridin-2-yl)-5-isopropyl-5-methyl-3,5-dihydro-4H-imidazol-4-one wild-type, pH 7.5, 37°C Nicotiana tabacum
0.00301
-
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide mutant K299Q pH 7.5, 37°C Nicotiana tabacum
0.00527
-
N-(4,6-dimethylpyrimidin-2-yl)-5-methyl-6,7,8,8a-tetrahydro-5aH-cyclopenta[e][1,2,4]triazolo[1,5-a]pyrimidine-2-sulfonamide wild-type, pH 7.5, 37°C Nicotiana tabacum