Any feedback?
Literature summary for 2.2.1.6 extracted from
- Purification and properties of Salmonella typhimurium acetolactate synthase isozyme II from Escherichia coli HB101/pDU9 (1985), Biochemistry, 24, 4952-4959.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| Mg2+ | Km: 0.022 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 9800 | - |
2 * 9800 + 2 * 59000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 59000 | - |
2 * 9800 + 2 * 59000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
| 140000 | - |
gel filtration | Salmonella enterica subsp. enterica serovar Typhimurium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salmonella enterica subsp. enterica serovar Typhimurium | - |
isoenzyme II, recombinantly produced in Escherichia coli HB101/pDU9 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| oxygen-dependent inactivation of the enzyme complexed with thiamine diphosphate, FAD and magnesium acetolactate, no inactivation of the enzyme-FAD complex | Salmonella enterica subsp. enterica serovar Typhimurium |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoenzyme II, recombinantly produced | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 25.3 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, concentrated enzyme solution is indefinitely stable | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | 2-acetolactate + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 2 * 9800 + 2 * 59000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | contains bound FAD, 0.88 mol per mol of protomer of 69000 Da | Salmonella enterica subsp. enterica serovar Typhimurium | |
| thiamine diphosphate | required as cofactor | Salmonella enterica subsp. enterica serovar Typhimurium | |
| thiamine diphosphate | Km: 0.0015 mM | Salmonella enterica subsp. enterica serovar Typhimurium | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
