Any feedback?
Literature summary for 2.2.1.5 extracted from
- Influence of allosteric regulators on individual steps in the reaction catalyzed by Mycobacterium tuberculosis 2-hydroxy-3-oxoadipate synthase (2013), J. Biol. Chem., 288, 21688-21702.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) CodonPlus-RILP cells | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GarA protein | non-competitive inhibition | Mycobacterium tuberculosis | |
| glyoxylate | - |
Mycobacterium tuberculosis |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.84 | - |
2-oxoglutarate | at pH 6.5 and 37°C | Mycobacterium tuberculosis | |
| 3.2 | - |
glyoxylate | at pH 6.5 and 37°C | Mycobacterium tuberculosis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Mycobacterium tuberculosis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + glyoxylate | Mycobacterium tuberculosis | - |
2-hydroxy-3-oxoadipate + CO2 | - |
? | |
| 2-oxoglutarate + glyoxylate | Mycobacterium tuberculosis H37Rv | - |
2-hydroxy-3-oxoadipate + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, Q Sepharose column chromatography, Mono Q column chromatography, and Sephacryl S-100 gel filtration | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + glyoxylate | - |
Mycobacterium tuberculosis | 2-hydroxy-3-oxoadipate + CO2 | - |
? | |
| 2-oxoglutarate + glyoxylate | - |
Mycobacterium tuberculosis H37Rv | 2-hydroxy-3-oxoadipate + CO2 | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (R)-HOA synthase | - |
Mycobacterium tuberculosis |
| HOA synthase | - |
Mycobacterium tuberculosis |
| HOAS | - |
Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.25 | - |
2-oxoglutarate | at pH 6.5 and 37°C | Mycobacterium tuberculosis | |
| 5.29 | - |
glyoxylate | at pH 6.5 and 37°C | Mycobacterium tuberculosis | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | - |
Mycobacterium tuberculosis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.36 | - |
GarA protein | at pH 6.5 and 37°C | Mycobacterium tuberculosis | |
| 53.1 | - |
glyoxylate | at pH 6.5 and 37°C | Mycobacterium tuberculosis | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1653 | - |
glyoxylate | at pH 6.5 and 37°C | Mycobacterium tuberculosis | |
| 5060 | - |
2-oxoglutarate | at pH 6.5 and 37°C | Mycobacterium tuberculosis | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
