Cloned (Comment) | Organism |
---|---|
expression of the catalytic trimer in Escherichia coli strain EK1911, which has a deletion in the pyrBI region of the chromosome and contains plasmids pEK406 coding for the Methanococcus jannaschii ATCase catalytic chain and pSJS1240 | Methanocaldococcus jannaschii |
expression of the regulatiry subunit in Escherichia coli ATCase-deficient derivative strain C600 which contains T7 RNA polymerase gene under lacUV5 control as a lambda lysogen | Methanocaldococcus jannaschii |
Crystallization (Comment) | Organism |
---|---|
catalytic chain in the presence of the regulatory chain in the hexagonal space group P6322, with one monomer per asymmetric unit, sitting drop vapor diffusion method, mixing of 0.0013 protein-ligand solution, containing 11 mg/ml protein in 50 mM Tris, pH 8.3, 150 mM NaCl, 2 mM BME, 0.05 mM zinc acetate, with 0.001 ml of reservoir solution containing 2.0 M ammonium sulfate, 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dihydrate, pH 5.6, or 2.0 M ammonium sulfate, 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M Tris-HCl, pH 7.5, 22°C, X-ray diffraction structure determination and analysis at 2.50 A resolution, molecular replacement | Methanocaldococcus jannaschii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + carbamoyl phosphate | Methanocaldococcus jannaschii | - |
phosphate + N-carbamoyl-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | Q58801 | regulatory subunit | - |
Methanocaldococcus jannaschii | Q58976 | catalytic subunit trimer | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-aspartate + carbamoyl phosphate | - |
Methanocaldococcus jannaschii | phosphate + N-carbamoyl-L-aspartate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
aspartate transcarbamoylase | - |
Methanocaldococcus jannaschii |
ATCase | - |
Methanocaldococcus jannaschii |