Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Aquifex aeolicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.55 | - |
Carbamoyl phosphate | pH 8.0, 37°C | Aquifex aeolicus | |
1 | - |
L-aspartate | pH 8.0, 37°C | Aquifex aeolicus | |
1.12 | - |
L-aspartate | pH 8.0, 75°C | Aquifex aeolicus | |
1.26 | - |
Carbamoyl phosphate | pH 8.0, 75°C | Aquifex aeolicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33557 | - |
3 * 37000, SDS-PAGE, recombinant enzyme with His-tag, 3 * 33557, calculated | Aquifex aeolicus |
37000 | - |
3 * 37000, SDS-PAGE, recombinant enzyme with His-tag, 3 * 33557, calculated | Aquifex aeolicus |
112800 | - |
gel filtration | Aquifex aeolicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aquifex aeolicus | - |
expression in Escherichia coli | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Aquifex aeolicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-aspartate | - |
Aquifex aeolicus | phosphate + N-carbamoyl-L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | channeling and transient complex formation between enzyme and carbamoyl phosphate synthetase | Aquifex aeolicus |
trimer | 3 * 37000, SDS-PAGE, recombinant enzyme with His-tag, 3 * 33557, calculated | Aquifex aeolicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
stable up to | Aquifex aeolicus |
87 | - |
5 min, 50% inactivation | Aquifex aeolicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.6 | - |
L-aspartate | pH 8.0, 37°C | Aquifex aeolicus | |
41.7 | - |
L-aspartate | pH 8.0, 75°C | Aquifex aeolicus |