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Literature summary for 2.1.3.2 extracted from

  • Purcarea, C.; Ahuja, A.; Lu, T.; Kovari, L.; Guy, H.I.; Evans, D.R.
    Aquifex aeolicus aspartate transcarbamoylase, an enzyme specialized for the efficient utilization of unstable carbamoyl phosphate at elevated temperature (2003), J. Biol. Chem., 278, 52924-52934.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Aquifex aeolicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.55
-
Carbamoyl phosphate pH 8.0, 37°C Aquifex aeolicus
1
-
L-aspartate pH 8.0, 37°C Aquifex aeolicus
1.12
-
L-aspartate pH 8.0, 75°C Aquifex aeolicus
1.26
-
Carbamoyl phosphate pH 8.0, 75°C Aquifex aeolicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33557
-
3 * 37000, SDS-PAGE, recombinant enzyme with His-tag, 3 * 33557, calculated Aquifex aeolicus
37000
-
3 * 37000, SDS-PAGE, recombinant enzyme with His-tag, 3 * 33557, calculated Aquifex aeolicus
112800
-
gel filtration Aquifex aeolicus

Organism

Organism UniProt Comment Textmining
Aquifex aeolicus
-
expression in Escherichia coli
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Aquifex aeolicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
Aquifex aeolicus phosphate + N-carbamoyl-L-aspartate
-
?

Subunits

Subunits Comment Organism
More channeling and transient complex formation between enzyme and carbamoyl phosphate synthetase Aquifex aeolicus
trimer 3 * 37000, SDS-PAGE, recombinant enzyme with His-tag, 3 * 33557, calculated Aquifex aeolicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
stable up to Aquifex aeolicus
87
-
5 min, 50% inactivation Aquifex aeolicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.6
-
L-aspartate pH 8.0, 37°C Aquifex aeolicus
41.7
-
L-aspartate pH 8.0, 75°C Aquifex aeolicus