Activating Compound | Comment | Organism | Structure |
---|---|---|---|
N-(Phosphonoacetyl)-L-aspartate | at concentrations below 0.000002 mM, inhibition above | Pseudomonas aeruginosa |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
hanging drop method, equal volumes of 1 mg/ml enzyme in 100 mM Tris-HCl, pH 8.5 is mixed with 100 mM citrate, pH 5, and 10% 6K polyethylene glycol | Pseudomonas aeruginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | excess MgCl2 abolishes inhibition | Pseudomonas aeruginosa | |
CTP | - |
Pseudomonas aeruginosa | |
GTP | - |
Pseudomonas aeruginosa | |
UTP | - |
Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.49 | - |
Carbamoyl phosphate | - |
Pseudomonas aeruginosa | |
2.6 | - |
L-aspartate | - |
Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
6 * 45000 + 6 * 36000, SDS-PAGE | Pseudomonas aeruginosa |
45000 | - |
6 * 45000 + 6 * 36000, SDS-PAGE | Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoylphosphate + L-aspartate | Pseudomonas aeruginosa | - |
phosphate + N-carbamoyl-L-aspartate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, Ni2+-affinity column | Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
450 | - |
recombinant enzyme | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
carbamoyl phosphate + L-aspartate | - |
Pseudomonas aeruginosa | phosphate + N-carbamoyl-L-aspartate | - |
? | |
carbamoylphosphate + L-aspartate | - |
Pseudomonas aeruginosa | phosphate + N-carbamoyl-L-aspartate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dodecamer | 6 * 45000 + 6 * 36000, SDS-PAGE | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | steep increase in activity until pH 8.5, decrase above | Pseudomonas aeruginosa |