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Literature summary for 2.1.3.2 extracted from
- Wheat germ aspartate transcarbamoylase: revised purification, stability and re-evaluation of regulatory kinetics in terms of the Monod-Wyman-Changeux model (1999), Eur. J. Biochem., 259, 71-78.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| UMP | - |
Triticum aestivum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.023 | - |
Carbamoyl phosphate | in the absence of UMP | Triticum aestivum | |
| 0.16 | - |
L-aspartate | - |
Triticum aestivum | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoylphosphate + L-aspartate | Triticum aestivum | - |
phosphate + N-carbamoyl-L-aspartate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Triticum aestivum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ethanol, 10-carboxydecyl-Sepharose, DEAE-cellulose | Triticum aestivum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.21 | - |
- |
Triticum aestivum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM Tris-HCl, pH 7.5, 25% glycerol, 3 mM sodium azide, indefinitely, no loss of activity | Triticum aestivum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + L-aspartate | - |
Triticum aestivum | phosphate + N-carbamoyl-L-aspartate | - |
? | |
| carbamoylphosphate + L-aspartate | - |
Triticum aestivum | phosphate + N-carbamoyl-L-aspartate | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 47.2 | - |
Carbamoyl phosphate | - |
Triticum aestivum | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00049 | - |
UMP | - |
Triticum aestivum | |
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Release 2023.1 (January 2023)
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