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Literature summary for 2.1.3.2 extracted from

  • Purcarea, C.; Erauso, G.; Prieur, D.; Herve, G.
    The catalytic and regulatory properties of aspartate transcarbamoylase from Pyrococcus abyssi, a new deep-sea hyperthermophilic archaeobacterium (1994), Microbiology, 140, 1967-1975.
No PubMed abstract available
Search for more literature for 2.1.3.2

Activating Compound

Activating Compound Comment Organism Structure
ATP
-
 Pyrococcus abyssi
N-(Phosphonoacetyl)-L-aspartate at concentrations below 0.001 mM Pyrococcus abyssi
succinate low concentrations, 35% activation in the presence of 2 mM aspartate Pyrococcus abyssi

Inhibitors

Inhibitors Comment Organism Structure
CTP
-
 Pyrococcus abyssi
additional information 50% inhibition at 80 MPa; not inhibited by phosphonoacetate, diphosphate or phosphate Pyrococcus abyssi
N-(Phosphonoacetyl)-L-aspartate 0.011 mM, 50% inhibition, low concentrations activate Pyrococcus abyssi
UTP
-
 Pyrococcus abyssi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information sigmoidal saturation curve for carbamoylphosphate and aspartate Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
carbamoylphosphate + L-aspartate Pyrococcus abyssi
-
 phosphate + N-carbamoyl-L-aspartate
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ion-exchange, gel filtration Pyrococcus abyssi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carbamoyl phosphate + L-aspartate
-
 Pyrococcus abyssi phosphate + N-carbamoyl-L-aspartate
-
 ?
carbamoylphosphate + L-aspartate
-
 Pyrococcus abyssi phosphate + N-carbamoyl-L-aspartate
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 no loss of activity after 6 h Pyrococcus abyssi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Pyrococcus abyssi