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Literature summary for 2.1.3.2 extracted from
- Studies on plant aspartate transcarbamylase. Purification and properties of the enzyme from mung-bean (Phaseolus aureus) seedlings (1974), Eur. J. Biochem., 47, 15-22.
General Stability
| General Stability | Organism |
|---|---|
| purified enzyme is inactivated if stored frozen | Vigna radiata var. radiata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5'-UMP | 2'-UMP and 3'-UMP have no effect | Vigna radiata var. radiata |  |
| ATP | 1 mM, 40% inhibition | Vigna radiata var. radiata | |
| GTP | 1 mM, 20% inhibition | Vigna radiata var. radiata | |
| Maleate | - |
Vigna radiata var. radiata | |
| succinate | - |
Vigna radiata var. radiata | |
| UDP | 1 mM, 45% inhibition | Vigna radiata var. radiata | |
| UMP | 1 mM, 75% inhibition | Vigna radiata var. radiata | |
| UTP | 1 mM, 40% inhibition | Vigna radiata var. radiata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 128000 | - |
gel filtration, sedimentation data | Vigna radiata var. radiata |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoylphosphate + L-aspartate | Vigna radiata var. radiata | - |
phosphate + N-carbamoyl-L-aspartate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata var. radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| MnSO4, ammonium sulfate, DEAE-cellulose, Sephadex G-100 | Vigna radiata var. radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Vigna radiata var. radiata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.7 | - |
- |
Vigna radiata var. radiata |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0-4°C, 1-2 days | Vigna radiata var. radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| carbamoyl phosphate + L-aspartate | specific for L-aspartate | Vigna radiata var. radiata | phosphate + N-carbamoyl-L-aspartate | - |
? | |
| carbamoylphosphate + L-aspartate | - |
Vigna radiata var. radiata | phosphate + N-carbamoyl-L-aspartate | - |
? | |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 75 | approx. 70% of maximal activity at 30°C, approx. 30% of maximal activity at 75°C | Vigna radiata var. radiata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
5 min, no loss of activity | Vigna radiata var. radiata |
| 55 | - |
5 min, 20% loss of activity | Vigna radiata var. radiata |
| 60 | - |
5 min, 50% loss of activity | Vigna radiata var. radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
maxima at pH 8.0 and pH 10.2 | Vigna radiata var. radiata |
| 10.2 | - |
maxima at pH 8.0 and pH 10.2 | Vigna radiata var. radiata |
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