Application | Comment | Organism |
---|---|---|
drug development | the human enzyme is a potential target for cancer treatment | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant soluble enzyme overexpression in Escherichia coli strain BL21(DE3) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofolate | strong substrate inhibition | Homo sapiens | |
Thiosemicarbazide | - |
Homo sapiens | |
Thiosemicarbazide | - |
Plasmodium falciparum | |
Thiosemicarbazide | - |
Plasmodium vivax |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics, overview | Homo sapiens | |
0.0052 | - |
(6S)-tetrahydrofolate | pH 7.0, 25°C, recombinant enzyme, with L-serine in HEPES buffer | Homo sapiens | |
0.007 | - |
(6S)-tetrahydrofolate | pH 7.0, 25°C, recombinant enzyme, with L-serine in phosphate buffer | Homo sapiens | |
0.021 | - |
(6S)-tetrahydrofolate | pH 7.0, 25°C, recombinant enzyme, with D-serine | Homo sapiens | |
0.17 | - |
L-serine | pH 7.0, 25°C, recombinant enzyme, in phosphate buffer | Homo sapiens | |
0.18 | - |
L-serine | pH 7.0, 25°C, recombinant enzyme, in HEPES buffer | Homo sapiens | |
55 | - |
D-serine | pH 7.0, 25°C, recombinant enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | cytosolic isoform | Homo sapiens | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52020 | - |
4 * 52020, cytosolic isoform, sequence calculation, 4 * 53000, recombinant cytosolic isoform, SDS-PAGE | Homo sapiens |
53000 | - |
4 * 52020, cytosolic isoform, sequence calculation, 4 * 53000, recombinant cytosolic isoform, SDS-PAGE | Homo sapiens |
195000 | - |
cytosolic isoform, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-tetrahydrofolate + D-serine | Plasmodium falciparum | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
(6S)-tetrahydrofolate + D-serine | Plasmodium vivax | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
(6S)-tetrahydrofolate + L-serine | Plasmodium falciparum | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
(6S)-tetrahydrofolate + L-serine | Plasmodium vivax | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
(6S)-tetrahydrofolate + L-serine | Homo sapiens | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Plasmodium falciparum | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Plasmodium vivax | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Homo sapiens | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Plasmodium falciparum | - |
tetrahydrofolate + D-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Plasmodium vivax | - |
tetrahydrofolate + D-serine | - |
r | |
additional information | Plasmodium falciparum | Plasmodium SHMT can use D-serine and L-serine as a substrate | ? | - |
? | |
additional information | Plasmodium vivax | Plasmodium SHMT can use D-serine and L-serine as a substrate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P34896 | - |
- |
Plasmodium falciparum | - |
- |
- |
Plasmodium vivax | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 11.2fold from Escherichia coli strain BL21(DE3) to homogeneity by polyethyleneimine precipitation, anion exchange chromatography, and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(6S)-tetrahydrofolate + D-serine | - |
Plasmodium falciparum | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
(6S)-tetrahydrofolate + D-serine | - |
Plasmodium vivax | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
(6S)-tetrahydrofolate + D-serine | poor activity | Homo sapiens | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
(6S)-tetrahydrofolate + L-serine | - |
Plasmodium falciparum | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
(6S)-tetrahydrofolate + L-serine | - |
Plasmodium vivax | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
(6S)-tetrahydrofolate + L-serine | - |
Homo sapiens | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Plasmodium falciparum | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Plasmodium vivax | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Homo sapiens | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Plasmodium falciparum | tetrahydrofolate + D-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Plasmodium vivax | tetrahydrofolate + D-serine | - |
r | |
additional information | Plasmodium SHMT can use D-serine and L-serine as a substrate | Plasmodium falciparum | ? | - |
? | |
additional information | Plasmodium SHMT can use D-serine and L-serine as a substrate | Plasmodium vivax | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 52020, cytosolic isoform, sequence calculation, 4 * 53000, recombinant cytosolic isoform, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Plasmodium falciparum |
serine hydroxymethyltransferase | - |
Plasmodium vivax |
serine hydroxymethyltransferase | - |
Homo sapiens |
SHMT | - |
Plasmodium falciparum |
SHMT | - |
Plasmodium vivax |
SHMT | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Plasmodium falciparum |
25 | - |
assay at | Plasmodium vivax |
25 | - |
assay at | Homo sapiens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
unlike Plasmodium enzymes in which the activity is almost abolished at lower temperatures, the activity of hcSHMT still remains active below the temperature breakpoint | Homo sapiens |
additional information | - |
unlike Plasmodium enzymes in which the activity is almost abolished at lower temperatures, the activity of human cytosolic SHMT still remains active below the temperature breakpoint | Plasmodium falciparum |
additional information | - |
unlike Plasmodium enzymes in which the activity is almost abolished at lower temperatures, the activity of human cytosolic SHMT still remains active below the temperature breakpoint | Plasmodium vivax |
3 | 43 | activity range | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | 8 | L-serine | pH 7.0, 25°C, recombinant enzyme, in HEPES buffer | Homo sapiens | |
15 | - |
L-serine | pH 7.0, 25°C, recombinant enzyme, in phosphate buffer | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
recombinant enzyme | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
5,10-methylenetetrahydrofolate | - |
Plasmodium falciparum | |
5,10-methylenetetrahydrofolate | - |
Plasmodium vivax | |
5,10-methylenetetrahydrofolate | - |
Homo sapiens | |
pyridoxal 5'-phosphate | dependent on. The binding environment of PLP in human and Plasmodium enzymes is significantly different | Plasmodium falciparum | |
pyridoxal 5'-phosphate | dependent on. The binding environment of PLP in human and Plasmodium enzymes is significantly different | Plasmodium vivax | |
pyridoxal 5'-phosphate | dependent on. The binding environment of PLP in human and Plasmodium enzymes is significantly different | Homo sapiens | |
tetrahydrofolate | - |
Plasmodium falciparum | |
tetrahydrofolate | - |
Plasmodium vivax | |
tetrahydrofolate | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the alpha class of PLP-dependent enzymes. The ligand binding environment of enzymes SHMT from human and Plasmodium are different, overview | Plasmodium falciparum |
evolution | the enzyme belongs to the alpha class of PLP-dependent enzymes. The ligand binding environment of enzymes SHMT from human and Plasmodium are different, overview | Plasmodium vivax |
evolution | the enzyme belongs to the alpha class of PLP-dependent enzymes. The ligand binding environment of enzymes SHMT from human and Plasmodium are different, overview | Homo sapiens |
physiological function | the enzyme is essential for parasite viability | Plasmodium falciparum |
physiological function | the enzyme is essential for parasite viability | Plasmodium vivax |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.172 | - |
D-serine | pH 7.0, 25°C, recombinant enzyme | Homo sapiens | |
88 | - |
L-serine | pH 7.0, 25°C, recombinant enzyme, in phosphate buffer | Homo sapiens | |
155.5 | - |
L-serine | pH 7.0, 25°C, recombinant enzyme, in HEPES buffer | Homo sapiens |