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Literature summary for 2.1.2.1 extracted from

  • Pinthong, C.; Maenpuen, S.; Amornwatcharapong, W.; Yuthavong, Y.; Leartsakulpanich, U.; Chaiyen, P.
    Distinct biochemical properties of human serine hydroxymethyltransferase compared with the Plasmodium enzyme: implications for selective inhibition (2014), FEBS J., 281, 2570-2583.
    View publication on PubMed

Application

Application Comment Organism
drug development the human enzyme is a potential target for cancer treatment Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant soluble enzyme overexpression in Escherichia coli strain BL21(DE3) Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
tetrahydrofolate strong substrate inhibition Homo sapiens
Thiosemicarbazide
-
Homo sapiens
Thiosemicarbazide
-
Plasmodium falciparum
Thiosemicarbazide
-
Plasmodium vivax

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetics, overview Homo sapiens
0.0052
-
(6S)-tetrahydrofolate pH 7.0, 25°C, recombinant enzyme, with L-serine in HEPES buffer Homo sapiens
0.007
-
(6S)-tetrahydrofolate pH 7.0, 25°C, recombinant enzyme, with L-serine in phosphate buffer Homo sapiens
0.021
-
(6S)-tetrahydrofolate pH 7.0, 25°C, recombinant enzyme, with D-serine Homo sapiens
0.17
-
L-serine pH 7.0, 25°C, recombinant enzyme, in phosphate buffer Homo sapiens
0.18
-
L-serine pH 7.0, 25°C, recombinant enzyme, in HEPES buffer Homo sapiens
55
-
D-serine pH 7.0, 25°C, recombinant enzyme Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol cytosolic isoform Homo sapiens 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52020
-
4 * 52020, cytosolic isoform, sequence calculation, 4 * 53000, recombinant cytosolic isoform, SDS-PAGE Homo sapiens
53000
-
4 * 52020, cytosolic isoform, sequence calculation, 4 * 53000, recombinant cytosolic isoform, SDS-PAGE Homo sapiens
195000
-
cytosolic isoform, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(6S)-tetrahydrofolate + D-serine Plasmodium falciparum
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r
(6S)-tetrahydrofolate + D-serine Plasmodium vivax
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r
(6S)-tetrahydrofolate + L-serine Plasmodium falciparum
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r
(6S)-tetrahydrofolate + L-serine Plasmodium vivax
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r
(6S)-tetrahydrofolate + L-serine Homo sapiens
-
5,10-methylenetetrahydrofolate + glycine + H2O
-
r
5,10-methylenetetrahydrofolate + glycine + H2O Plasmodium falciparum
-
tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O Plasmodium vivax
-
tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O Homo sapiens
-
tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O Plasmodium falciparum
-
tetrahydrofolate + D-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O Plasmodium vivax
-
tetrahydrofolate + D-serine
-
r
additional information Plasmodium falciparum Plasmodium SHMT can use D-serine and L-serine as a substrate ?
-
?
additional information Plasmodium vivax Plasmodium SHMT can use D-serine and L-serine as a substrate ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens P34896
-
-
Plasmodium falciparum
-
-
-
Plasmodium vivax
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 11.2fold from Escherichia coli strain BL21(DE3) to homogeneity by polyethyleneimine precipitation, anion exchange chromatography, and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(6S)-tetrahydrofolate + D-serine
-
Plasmodium falciparum 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
(6S)-tetrahydrofolate + D-serine
-
Plasmodium vivax 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
(6S)-tetrahydrofolate + D-serine poor activity Homo sapiens 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
(6S)-tetrahydrofolate + L-serine
-
Plasmodium falciparum 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
(6S)-tetrahydrofolate + L-serine
-
Plasmodium vivax 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
(6S)-tetrahydrofolate + L-serine
-
Homo sapiens 5,10-methylenetetrahydrofolate + glycine + H2O
-
r
5,10-methylenetetrahydrofolate + glycine + H2O
-
Plasmodium falciparum tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O
-
Plasmodium vivax tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O
-
Homo sapiens tetrahydrofolate + L-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O
-
Plasmodium falciparum tetrahydrofolate + D-serine
-
r
5,10-methylenetetrahydrofolate + glycine + H2O
-
Plasmodium vivax tetrahydrofolate + D-serine
-
r
additional information Plasmodium SHMT can use D-serine and L-serine as a substrate Plasmodium falciparum ?
-
?
additional information Plasmodium SHMT can use D-serine and L-serine as a substrate Plasmodium vivax ?
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 52020, cytosolic isoform, sequence calculation, 4 * 53000, recombinant cytosolic isoform, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
serine hydroxymethyltransferase
-
Plasmodium falciparum
serine hydroxymethyltransferase
-
Plasmodium vivax
serine hydroxymethyltransferase
-
Homo sapiens
SHMT
-
Plasmodium falciparum
SHMT
-
Plasmodium vivax
SHMT
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Plasmodium falciparum
25
-
assay at Plasmodium vivax
25
-
assay at Homo sapiens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
unlike Plasmodium enzymes in which the activity is almost abolished at lower temperatures, the activity of hcSHMT still remains active below the temperature breakpoint Homo sapiens
additional information
-
unlike Plasmodium enzymes in which the activity is almost abolished at lower temperatures, the activity of human cytosolic SHMT still remains active below the temperature breakpoint Plasmodium falciparum
additional information
-
unlike Plasmodium enzymes in which the activity is almost abolished at lower temperatures, the activity of human cytosolic SHMT still remains active below the temperature breakpoint Plasmodium vivax
3 43 activity range Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2 8 L-serine pH 7.0, 25°C, recombinant enzyme, in HEPES buffer Homo sapiens
15
-
L-serine pH 7.0, 25°C, recombinant enzyme, in phosphate buffer Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
recombinant enzyme Homo sapiens

Cofactor

Cofactor Comment Organism Structure
5,10-methylenetetrahydrofolate
-
Plasmodium falciparum
5,10-methylenetetrahydrofolate
-
Plasmodium vivax
5,10-methylenetetrahydrofolate
-
Homo sapiens
pyridoxal 5'-phosphate dependent on. The binding environment of PLP in human and Plasmodium enzymes is significantly different Plasmodium falciparum
pyridoxal 5'-phosphate dependent on. The binding environment of PLP in human and Plasmodium enzymes is significantly different Plasmodium vivax
pyridoxal 5'-phosphate dependent on. The binding environment of PLP in human and Plasmodium enzymes is significantly different Homo sapiens
tetrahydrofolate
-
Plasmodium falciparum
tetrahydrofolate
-
Plasmodium vivax
tetrahydrofolate
-
Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the alpha class of PLP-dependent enzymes. The ligand binding environment of enzymes SHMT from human and Plasmodium are different, overview Plasmodium falciparum
evolution the enzyme belongs to the alpha class of PLP-dependent enzymes. The ligand binding environment of enzymes SHMT from human and Plasmodium are different, overview Plasmodium vivax
evolution the enzyme belongs to the alpha class of PLP-dependent enzymes. The ligand binding environment of enzymes SHMT from human and Plasmodium are different, overview Homo sapiens
physiological function the enzyme is essential for parasite viability Plasmodium falciparum
physiological function the enzyme is essential for parasite viability Plasmodium vivax

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.172
-
D-serine pH 7.0, 25°C, recombinant enzyme Homo sapiens
88
-
L-serine pH 7.0, 25°C, recombinant enzyme, in phosphate buffer Homo sapiens
155.5
-
L-serine pH 7.0, 25°C, recombinant enzyme, in HEPES buffer Homo sapiens