Cloned (Comment) | Organism |
---|---|
gene glyA, DNA and amino acid sequence determination and analysis, thermal asymmetric interlaced PCR, TAIL-PCR, sequence comparisons and phylogenetic analysis, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3) | Arthrobacter sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | strong inhibition at 1 mM | Arthrobacter sp. | |
CTAB | - |
Arthrobacter sp. | |
Cu2+ | strong inhibition at 1 mM | Arthrobacter sp. | |
Fe2+ | strong inhibition at 1 mM | Arthrobacter sp. | |
Hg2+ | strong inhibition at 1 mM | Arthrobacter sp. | |
Mn2+ | strong inhibition at 1 mM | Arthrobacter sp. | |
SDS | - |
Arthrobacter sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates slightly | Arthrobacter sp. | |
EDTA | activates slightly | Arthrobacter sp. | |
Mg2+ | activates slightly | Arthrobacter sp. | |
additional information | K+, Na+ and NH4+ show no appreciable impact on the SHMT activity | Arthrobacter sp. | |
Pb2+ | activates slightly | Arthrobacter sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Arthrobacter sp. | - |
tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | Arthrobacter sp. MCCC 1A05493 | - |
tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | Arthrobacter sp. | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
tetrahydrofolate + L-serine | Arthrobacter sp. MCCC 1A05493 | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | - |
gene glyA | - |
Arthrobacter sp. MCCC 1A05493 | - |
gene glyA | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Arthrobacter sp. | tetrahydrofolate + L-serine | - |
r | |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Arthrobacter sp. MCCC 1A05493 | tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | - |
Arthrobacter sp. | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r | |
tetrahydrofolate + L-serine | - |
Arthrobacter sp. MCCC 1A05493 | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Arthrobacter sp. |
SHMT | - |
Arthrobacter sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Arthrobacter sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
- |
Arthrobacter sp. |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the alpha-family of fold type I, and pyridoxal 5'-phosphate-dependent enzymes | Arthrobacter sp. |
physiological function | SHMT plays an important role in the assimilation of C1 compounds, yielding the main L-serine intermediate | Arthrobacter sp. |