Literature summary for 2.1.2.1 extracted from

Gagnon, D.; Foucher, A.; Girard, I.; Ouellette, M.
Stage specific gene expression and cellular localization of two isoforms of the serine hydroxymethyltransferase in the protozoan parasite Leishmania (2006), Mol. Biochem. Parasitol., 150, 63-71.

Search for more literature for 2.1.2.1

Application

Application	Comment	Organism
analysis	protein sequence of the two isoforms, the short form SHMT-S and the long form SHMT-L, show 59% identity	Leishmania major

Cloned(Commentary)

Cloned (Comment)	Organism
both isoforms cloned in frame with EGFP at their C-terminus and transfected into Leishmania major	Leishmania major

Inhibitors

Inhibitors	Comment	Organism	Structure
D-cycloserine	overexpression of SHMT-S increases resistance in a rich folate containing medium	Leishmania major
Thiosemicarbazide	poorly active against promastigotes, but SHMT-S transfectants can provide a small but significant resistance	Leishmania major

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	SHMT-S	Leishmania major	5829	-
additional information	SHMT-L in an organelle that has hallmarks of the parasite mitochondrion	Leishmania major	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
50600	-	sequence analysis, SHMT-S	Leishmania major
53100	-	sequence analysis, SHMT-L	Leishmania major

Organism

Organism	UniProt	Comment	Textmining
Leishmania infantum	-	-	-
Leishmania major	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
amastigote	preferentially expressed	Leishmania infantum	-
promastigote	-	Leishmania infantum	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-serine + tetrahydrofolate	adding 2fold more glycine in the medium increases significantly the expression of SHMT-S and to an even higher level, the expression of SHMT-L, adding 2fold more serine has the reverse effect on the expression of SHMT-L, while the expression of SHMT-S does not change significantly	Leishmania infantum	glycine + 5,10-methylenetetrahydrofolate + H2O	-	?

Synonyms

Synonyms	Comment	Organism
serine hydroxymethyltransferase	-	Leishmania major
serine hydroxymethyltransferase	-	Leishmania infantum
SHMT-L	-	Leishmania major
SHMT-L	-	Leishmania infantum
SHMT-S	-	Leishmania major
SHMT-S	-	Leishmania infantum

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Release 2023.1 (January 2023)