Application | Comment | Organism |
---|---|---|
analysis | SHMT1 is a zinc-inducible gene, provides first mechanism for the regulation of folate-mediated one-carbon metabolism by zinc | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
into the pGL3 vector and transfected into MCF-7 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of the consensus metal regulatory element sequence decreases the promoter activity of the -219 to -1 fragment by 60% in the absence of L-mimosine and attenuates L-mimosine inhibition by nearly 40%, mutation of the NF1 consensus sequence in the SHMT1 promoter -219 to -1 partially attenuates L-mimosine inhibition by ca. 20% without influencing SHMT1 promoter activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-mimosine | inhibits SHMT1 transcription by chelating zinc, eliminates the metal regulatory element- and Sp1-binding activity in nuclear extracts from MCF-7 cells, but not in nuclear extracts from the mimosine-resistant cell line, MCF-7/2a | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Homo sapiens | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zinc | within a mimosine-responsive transcriptional element localized within the first 50 base pairs of the human SHMT1 promoter, 50-base-pair sequence contains a consensus zinc-sensing metal regulatory element at position -44 to -38 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MCF-7 cell | - |
Homo sapiens | - |
MCF-7/2a cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Homo sapiens |
SHMT1 | - |
Homo sapiens |