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Literature summary for 2.1.2.1 extracted from

  • Jala, V.R.; Ambili, M.; Prakash, V.; Rao, N.A.; Savithri, H.S.
    Disruption of distal interactions of Arg262 and of substrate binding to Ser52 affect catalysis of sheep liver cytosolic serine hydroxymethyltransferase (2003), Indian J. Biochem. Biophys., 40, 226-237.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes S52A, S52C and R262A are overexpressed in Escherichia coli Ovis aries

Protein Variants

Protein Variants Comment Organism
R262A KM-value for L-Ser is 5.2fold higher than the wild-type value, the turnover-number is 5.25fold lower than the wild-type value, no transamination reaction with D-Ala, reaction with L-allo-Thr is 20fold decreased compared to wild-type value. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme Ovis aries
S52A KM-value for L-Ser is 1.3fold higher than the wild-type value, the turnover-number is 6fold lower than the wild-type value, transamination reaction with D-Ala is 20% of the wild-type activity, reaction with L-allo-Thr is 15% of the wild-type activity. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme Ovis aries
S52C KM-value for L-Ser is 11.2fold higher than the wild-type value, the turnover-number is 21fold lower than the wild-type value, no transamination reaction with D-Ala, no reaction with L-allo-Thr. L-Ser is unable to enhance the thermal stability as it does in wild-type enzyme Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
L-Ser pH 7.4, 37°C, wild-type enzyme Ovis aries
1.3
-
L-Ser pH 7.4, 37°C, mutant enzyme S52A Ovis aries
5.2
-
L-Ser pH 7.4, 37°C, mutant enzyme R262A Ovis aries
11.2
-
L-Ser pH 7.4, 37°C, mutant enzyme S52C Ovis aries

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Ovis aries 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
53000
-
4 * 53000, SDS-PAGE Ovis aries
210000
-
gel filtration Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,6,7,8-tetrahydrofolate + L-Ser
-
Ovis aries 5,10-methylenetetrahydrofolate + glycine + H2O
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 53000, SDS-PAGE Ovis aries

Synonyms

Synonyms Comment Organism
SHMT
-
Ovis aries

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
Tm-value for wild-type enzyme and mutant enzymes R262A, S52C and S52A without ligands or with Gly as ligand. Tm-value for mutant enzymes mutant enzymes R262A, S52C and S52A with L-Ser as ligand Ovis aries
68
-
Tm-value for wild-type enzyme with L-Ser as ligand Ovis aries

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
L-Ser pH 7.4, 37°C, mutant enzyme S52C Ovis aries
0.7
-
L-Ser pH 7.4, 37°C, mutant enzyme S52A Ovis aries
0.8
-
L-Ser pH 7.4, 37°C, mutant enzyme R262A Ovis aries
4.2
-
L-Ser pH 7.4, 37°C, wild-type enzyme Ovis aries