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Literature summary for 2.1.2.1 extracted from

  • Jala, V.R.; Prakash, V.; Rao, N.A.; Savithri, H.S.
    Overexpression and characterization of dimeric and tetrameric forms of recombinant serine hydroxymethyltransferase from Bacillus stearothermophilus (2002), J. Biosci., 27, 233-242.
    View publication on PubMed
Search for more literature for 2.1.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
 L-serine only tetrameric form Geobacillus stearothermophilus
0.9
-
 L-serine only dimeric form Geobacillus stearothermophilus
1
-
 L-serine
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 gel filtration Geobacillus stearothermophilus
90000
-
 dimeric form Geobacillus stearothermophilus
180000
-
 tetrameric form, gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + tetrahydrofolate Geobacillus stearothermophilus
-
 glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
gene cloned and expressed in Escherichia coli Geobacillus stearothermophilus

Reaction

Reaction Comment Organism Reaction ID
5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine mechanism Geobacillus stearothermophilus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1
-
 only tetrameric form Geobacillus stearothermophilus
4.2
-
-
 Geobacillus stearothermophilus
6.7
-
 only dimeric form Geobacillus stearothermophilus
32.4
-
 dimeric form at 80°C Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
 Geobacillus stearothermophilus glycine + 5,10-methylenetetrahydrofolate + H2O
-
 ?
additional information enzyme catalyses the racemization of D- and L-alanine Geobacillus stearothermophilus ?
-
 ?

Subunits

Subunits Comment Organism
More 90% dimer and 10% tetramer in the same organism, one subunit: 45000 Da, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
 dimeric form of enzyme Geobacillus stearothermophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 Tm values of enzyme and dimeric and tetrameric forms in the absence and presence of ligands Geobacillus stearothermophilus
additional information
-
 pyridoxal 5'-phosphate increases the thermal stability Geobacillus stearothermophilus
additional information
-
 L-serine increases the thermal stability Geobacillus stearothermophilus
60
-
 enzyme stable in the absence of any ligand Geobacillus stearothermophilus
70
-
 50% loss of activity in the absence of any ligand Geobacillus stearothermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6
-
 L-serine only tetrameric form Geobacillus stearothermophilus
4.3
-
 L-serine
-
 Geobacillus stearothermophilus
5
-
 L-serine only dimeric form Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Geobacillus stearothermophilus