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Literature summary for 2.1.2.1 extracted from

  • Trivedi, V.; Gupta, A.; Jala, V.R.; Saravanan, P.; Rao, G.S.J.; Rao, N.A.; Savithri, H.S.; Subramanya, H.S.
    Crystal structure of binary and ternary complexes of serine hydroxymethyltransferase from Bacillus stearothermophilus: insights into the catalytic mechanism (2002), J. Biol. Chem., 277, 17161-17169.
    View publication on PubMed

Application

Application Comment Organism
medicine enzyme is a potential target for cancer chemotherapy Geobacillus stearothermophilus

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus stearothermophilus

Crystallization (Commentary)

Crystallization (Comment) Organism
internal aldimine form, external aldimine form with bound serine and glycine, ternary complex with glycine and 5-formyl tetrahydrofolate Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-serine + tetrahydrofolate Geobacillus stearothermophilus
-
glycine + 5,10-methylenetetrahydrofolate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Geobacillus stearothermophilus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine evidence for a direct transfer mechanism for the enzyme catalysed reaction Geobacillus stearothermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-serine + tetrahydrofolate
-
Geobacillus stearothermophilus glycine + 5,10-methylenetetrahydrofolate + H2O
-
?
L-serine + tetrahydrofolate
-
Geobacillus stearothermophilus glycine + 5,10-methylenetetrahydrofolate + H2O
-
r
additional information enzyme catalyses the racemization of D- and L-alanine Geobacillus stearothermophilus ?
-
?

Subunits

Subunits Comment Organism
dimer gel filtration, crystallographic symmetry Geobacillus stearothermophilus

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Geobacillus stearothermophilus