Application | Comment | Organism |
---|---|---|
medicine | enzyme is a potential target for cancer chemotherapy | Ovis aries |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Ovis aries |
Protein Variants | Comment | Organism |
---|---|---|
E74K | specific activities drastically reduced with serine as substrate, but D-alanine transamination and allothreonine cleavage at rates comparable with wild-type enzyme | Ovis aries |
E74Q | specific activities drastically reduced with serine as substrate, but D-alanine transamination and allothreonine cleavage at rates comparable with wild type enzyme | Ovis aries |
Y82F | 95% loss of activity, kcat and Km decreased, a role in stabilizing the quinonoid intermediate | Ovis aries |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | 0.9 | L-serine | mutant enzyme Y82F | Ovis aries | |
1 | - |
L-serine | - |
Ovis aries | |
1.5 | - |
L-serine | mutant enzyme E74Q | Ovis aries |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
4 * 53000, mutant enzyme | Ovis aries |
220000 | - |
mutant enzyme | Ovis aries |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | Ovis aries | - |
glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine | mechanism | Ovis aries |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
mutant enzyme E74Q | Ovis aries |
0.16 | - |
mutant enzyme Y82F | Ovis aries |
4.2 | - |
- |
Ovis aries |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Ovis aries | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
additional information | enzyme catalyzes the pyridoxal 5'-phosphate dependent reversible cleavage of 3-hydroxy-alpha-amino acids | Ovis aries | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | removal of the bound pyridoxal 5'-phosphate from the mutant tetrameric enzyme leads to dissociation to a dimer | Ovis aries |
homotetramer | 4 * 53000, mutant enzyme | Ovis aries |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
thermograms of enzyme and mutant enzyme in the absence and presence of L-serine | Ovis aries |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
Tm: 55°C, increased to 65°C in the presence of L-serine, Tm: 68°C mutant enzyme Y82F in the presence of serine | Ovis aries |
additional information | - |
L-serine increases the thermal stability | Ovis aries |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
L-serine | mutant enzyme E74Q | Ovis aries | |
0.0967 | - |
L-serine | mutant enzyme Y82F | Ovis aries | |
4.1 | - |
L-serine | - |
Ovis aries |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Ovis aries |