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Literature summary for 2.1.2.1 extracted from
- Purification and properties of mitochondrial serine hydroxymethyltransferase (1980), J. Biol. Chem., 255, 7801-7806.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| mitochondrial enzyme | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Antibodies to cytosolic enzyme | - |
Oryctolagus cuniculus | |
| Antibodies to mitochondrial enzyme | no inhibition of cytosolic enzyme | Oryctolagus cuniculus | |
| L-cysteine | - |
Oryctolagus cuniculus |  |
| L-serine | competitive to glycine | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | 0.046 | tetrahydrofolate | - |
Oryctolagus cuniculus | |
| 1.4 | - |
L-serine | - |
Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Oryctolagus cuniculus | 5737 | - |
| mitochondrion | - |
Oryctolagus cuniculus | 5739 | - |
| additional information | comparison of cytosolic and mitochondrial forms of enzyme | Oryctolagus cuniculus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
4 * 53000, SDS-PAGE | Oryctolagus cuniculus |
| 215000 | - |
gel filtration, amino acid composition | Oryctolagus cuniculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-serine + tetrahydrofolate | - |
Oryctolagus cuniculus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 53000, SDS-PAGE | Oryctolagus cuniculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Oryctolagus cuniculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| tetrahydrofolate | requirement with L-serine or L-2-methylserine as substrate | Oryctolagus cuniculus | |
| tetrahydrofolate | allosteric regulation | Oryctolagus cuniculus | |
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