Cloned (Comment) | Organism |
---|---|
PpSABATH1 full-length cDNA cloned into the pET100/DTOPO vector in Escherichia coli | Physcomitrium patens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 5 mM chloride salt, mild inhibitory effect, <25%, PpSABATH1 | Physcomitrium patens | |
Cu2+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens | |
Fe2+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens | |
Fe3+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens | |
Mg2+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens | |
Mn2+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens | |
Zn2+ | 5 mM chloride salt, strong inhibitory effect, 50-100%, PpSABATH1 | Physcomitrium patens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83.2 | - |
thiobenzoic acid | PpSABATH1, pH 7.5, 25°C | Physcomitrium patens | |
94.2 | - |
S-adenosyl-L-methionine | PpSABATH1, pH 7.5, 25°C | Physcomitrium patens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | 5 mM chloride salt, stimulates PpSABATH1 activity by 70% | Physcomitrium patens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Physcomitrium patens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
purification of recombinant His-tagged PpSABATH1 protein | Physcomitrium patens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0696 | - |
with thiobenzoic acid as substrate, pH 7.5, 25°C | Physcomitrium patens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dithiothreitol + S-adenosyl-L-methionine | - |
Physcomitrium patens | ? | - |
? | |
furfuryl thiol + S-adenosyl-L-methionine | - |
Physcomitrium patens | ? | - |
? | |
thiobenzoic acid + S-adenosyl-L-methionine | - |
Physcomitrium patens | methylthiobenzoate + S-adenosyl-L-homocysteine + H+ | - |
? | |
thiophenol + S-adenosyl-L-methionine | - |
Physcomitrium patens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PpSABATH1 | - |
Physcomitrium patens |
SABATH methyltransferase | - |
Physcomitrium patens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | - |
loss of 20% of enzyme activity when incubated for 30 min, PpSABATH1 | Physcomitrium patens |
20 | - |
loss of 57% of enzyme activity when incubated for 30 min, PpSABATH1 | Physcomitrium patens |
30 | - |
loss of 64% of enzyme activity when incubated for 30 min, PpSABATH1 | Physcomitrium patens |
50 | - |
loss of 100% of enzyme activity when incubated for 30 min, PpSABATH1 | Physcomitrium patens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.046 | - |
thiobenzoic acid | PpSABATH1, pH 7.5, 25°C | Physcomitrium patens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
PpSABATH1 | Physcomitrium patens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
70% of its maximal enzyme activity, PpSABATH1 | Physcomitrium patens |
8 | - |
54% of its maximal enzyme activity, PpSABATH1 | Physcomitrium patens |
9 | - |
33% of its maximal enzyme activity, PpSABATH1 | Physcomitrium patens |
Organism | Comment | Expression |
---|---|---|
Physcomitrium patens | 2 h after treatment with 250 microM and 500 microM thiobenzoic acid expression of PpSABATH1 is down-regulated | down |
Physcomitrium patens | 24 h after treatment, expression of PpSABATH1 is up-regulated by eight-fold in plants treated with 250 microM thiobenzoic acid and 16-fold in plants treated with 500 microM thiobenzoic acid | up |