Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli Rosetta II (DE3) | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
co-crystallized with S-adenosyl-L-homocysteine | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
V23C | not conserved active site residue, slightly improved activity, lowered stability | Arabidopsis thaliana |
Y172F | reduced efficiency with Cl- | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.099 | - |
NCS- | wild type protein, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.103 | - |
NCS- | V23C mutant protein, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana | |
0.141 | - |
NCS- | Y172F mutant protein, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q0WP12 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography, His-tag remove | Arabidopsis thaliana |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0434 | - |
substrate NCS-, V23C mutant protein, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
0.0436 | - |
substrate NCS-, wild type protein, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
0.046 | - |
substrate NCS-, Y172F mutant protein, pH not specified in the publication, temperature not specified in the publication | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiocyanate + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | methyl thiocyanate + S-adenosyl-L-homocysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SAM-dependent halide methyltransferase | - |
Arabidopsis thaliana |