Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Methanocaldococcus jannaschii |
expressed in Escherichia coli BL21(DE3) cells | Methanothermus fervidus |
Crystallization (Comment) | Organism |
---|---|
in complex with either hydroxocobalamin or methylcobalamin, vapor diffusion method, using 26% (w/v) PEG 3000, 100 mM Na citrate pH 5.5 | Methanothermus fervidus |
in complex with either hydroxocobalamin or methylcobalamin, vapor diffusion method, using 26% (w/v) PEG 3000, 2.2 M DL-malate pH 7.6, 100 mM Tris-HCl pH 8.0 | Methanocaldococcus jannaschii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Methanothermus fervidus | 5737 | - |
membrane | - |
Methanocaldococcus jannaschii | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in | Methanocaldococcus jannaschii | - |
5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out | - |
? | |
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in | Methanothermus fervidus | - |
5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out | - |
? | |
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in | Methanothermus fervidus ATCC 43054 | - |
5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Methanothermus fervidus | E3GWY7 | - |
- |
Methanothermus fervidus ATCC 43054 | E3GWY7 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ -charged HiTrap column chromatography | Methanocaldococcus jannaschii |
Ni2+ -charged HiTrap column chromatography | Methanothermus fervidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in | - |
Methanocaldococcus jannaschii | 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out | - |
? | |
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in | - |
Methanothermus fervidus | 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out | - |
? | |
5-methyl-5,6,7,8-tetrahydromethanopterin + CoM + 2 Na+/in | - |
Methanothermus fervidus ATCC 43054 | 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na+/out | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CH3-H4MPT: CoM-SH methyltransferase complex | - |
Methanocaldococcus jannaschii |
CH3-H4MPT: CoM-SH methyltransferase complex | - |
Methanothermus fervidus |
methyl-tetrahydromethanopterin:coenzyme M methyltransferase complex | - |
Methanocaldococcus jannaschii |
methyl-tetrahydromethanopterin:coenzyme M methyltransferase complex | - |
Methanothermus fervidus |
MtrA-H complex | - |
Methanocaldococcus jannaschii |
MtrA-H complex | - |
Methanothermus fervidus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Cobalamin | - |
Methanocaldococcus jannaschii | |
Cobalamin | - |
Methanothermus fervidus |