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Literature summary for 2.1.1.77 extracted from
- Protein L-isoaspartyl methyltransferase2 is differentially expressed in chickpea and enhances seed vigor and longevity by reducing abnormal isoaspartyl accumulation predominantly in seed nuclear proteins (2013), Plant Physiol., 161, 1141-1157.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Arabidopsis thaliana | Cicer arietinum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.006 | - |
S-adenosyl-L-methionine | isoform PIMT2, at 37°C and pH 8.0 | Cicer arietinum |  |
| 0.0065 | - |
S-adenosyl-L-methionine | isoform PIMT1, at 37°C and pH 9.0 | Cicer arietinum | |
| 0.208 | - |
Val-Tyr-Pro(L-iso-aspartate)-His-Ala | isoform PIMT2, at 37°C and pH 8.0 | Cicer arietinum | |
| 0.27 | - |
Val-Tyr-Pro(L-iso-aspartate)-His-Ala | isoform PIMT1, at 37°C and pH 9.0 | Cicer arietinum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | isoform PIMT1 | Cicer arietinum | 5829 | - |
| nucleus | isoform PIMT2 | Cicer arietinum | 5634 | - |
| nucleus | isoform PIMT2' | Cicer arietinum | 5634 | - |
| plasma membrane | isoform PIMT2 | Cicer arietinum | 5886 | - |
| plasma membrane | isoform PIMT2' | Cicer arietinum | 5886 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 25000 | - |
x * 25000, isoform PIMT1, SDS-PAGE | Cicer arietinum |
| 31000 | - |
x * 31000, isoform PIMT2, SDS-PAGE | Cicer arietinum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + protein L-isoaspartate | Cicer arietinum | - |
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cicer arietinum | - |
variant BGD72 | - |
| Cicer arietinum | M4I6P1 | isoform PIMT2'; variant BGD72 | - |
| Cicer arietinum | M4I787 | isoform PIMT2; variant BGD72 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Cicer arietinum | - |
| root | - |
Cicer arietinum | - |
| seed | - |
Cicer arietinum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| S-adenosyl-L-methionine + protein L-isoaspartate | - |
Cicer arietinum | S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester | - |
? | |
| S-adenosyl-L-methionine + Val-Tyr-Pro(L-iso-aspartate)-His-Ala | - |
Cicer arietinum | S-adenosyl-L-homocysteine + Val-Tyr-Pro(L-isoaspartate alpha-methyl ester)-His-Ala | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 25000, isoform PIMT1, SDS-PAGE | Cicer arietinum |
| ? | x * 31000, isoform PIMT2, SDS-PAGE | Cicer arietinum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PIMT1 | isoform | Cicer arietinum |
| PIMT2 | isoform | Cicer arietinum |
| PIMT2' | isoform | Cicer arietinum |
| protein L-isoaspartyl methyltransferase2 | - |
Cicer arietinum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
maximum activity at 50°C. At temperatures above 60°C, enzyme activity shows a sharp decline | Cicer arietinum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | maximum enzyme activity at pH 6.0 to 8.0, while the enzyme activity is significantly inhibited above pH 9.5 | Cicer arietinum |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Cicer arietinum | isoform PIMT1 transcript accumulation is significantly greater in abscisic acid-treated seedlings than in the control | up |
| Cicer arietinum | isoform PIMT2 transcript accumulates about 4fold more relative to the control under environmental stress conditions like treatment with polyethylene glycol, 200 mM sodium chloride or 0.0005 mM paraquat. Isoform PIMT2 transcript accumulation is significantly greater in abscisic acid-treated seedlings than in the control | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | isoform PIMT1 enhances seed vigor and longevity by repairing such abnormal proteins mainly in the cytosolic fraction | Cicer arietinum |
| physiological function | isoform PIMT2 enhances seed vigor and longevity by repairing abnormal isoaspartyl residues predominantly in nuclear proteins upon seed-specific expression | Cicer arietinum |
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Release 2023.1 (January 2023)
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