Literature summary for 2.1.1.77 extracted from

Bennett, E.J.; Bjerregaard, J.; Knapp, J.E.; Chavous, D.A.; Friedman, A.M.; Royer, W.E., Jr.; O'Connor, C.M.
Catalytic implications from the Drosophila protein L-isoaspartyl methyltransferase structure and site-directed mutagenesis (2003), Biochemistry, 42, 12844-12853.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in transgenic flies extends the normal life span, suggesting that protein damage can be a limiting factor in longevity	Drosophila melanogaster

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapour diffusion method, resolution of 2.2 A	Drosophila melanogaster

Protein Variants

Protein Variants	Comment	Organism
S60A	inactive mutant protein	Drosophila melanogaster
S60Q	the ratio of turnover number to KM-value for ovalbumin L-isoaspartate is 24.3fold lower than the wild-type value, the ratio of turnover-number to KM-value for KASA(iso-D)LAKY is 21fold lower than wild-type value	Drosophila melanogaster
S60T	the ratio of turnover number to KM-value for ovalbumin L-isoaspartate is 6.2fold lower than the wild-type value, the ratio of turnover-number to KM-value for KASA(iso-D)LAKY is 2fold lower than the wild-type value	Drosophila melanogaster

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0145	-	KASA(iso-D)LAKY	wild-type enzyme	Drosophila melanogaster
0.023	-	KASA(iso-D)LAKY	mutant enzyme S60Q	Drosophila melanogaster
0.0286	-	KASA(iso-D)LAKY	mutant enzyme S60T	Drosophila melanogaster
0.0423	-	ovalbumin L-isoaspartate	mutant enzyme S60Q	Drosophila melanogaster
0.121	-	ovalbumin L-isoaspartate	wild-type enzyme	Drosophila melanogaster
0.159	-	ovalbumin L-isoaspartate	mutant enzyme S60T	Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + protein L-isoaspartate	Drosophila melanogaster	the enzyme catalyzes the methylation of L-isoaspartyl residues that arise spontaneously in proteins with age, thereby initiating a repair process that restores the normal backbone configuration to the damaged polypeptide	S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester	-	?

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	Q27869	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Drosophila melanogaster

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + KASA(iso-D)LAKY	-	Drosophila melanogaster	S-adenosyl-L-homocysteine + KASA(isoaspartate alpha-methyl ester)LAKY	-	?
S-adenosyl-L-methionine + KASA(iso-D)LAKY	-	Drosophila melanogaster	S-adenosyl-L-homocysteine + KASA(L-isoaspartate alpha-methyl ester)LAKY	-	?
S-adenosyl-L-methionine + ovalbumin L-isoaspartate	-	Drosophila melanogaster	S-adenosyl-L-homocysteine + ovalbumin L-isoaspartate alpha-methyl ester	-	?
S-adenosyl-L-methionine + protein L-isoaspartate	the enzyme catalyzes the methylation of L-isoaspartyl residues that arise spontaneously in proteins with age, thereby initiating a repair process that restores the normal backbone configuration to the damaged polypeptide	Drosophila melanogaster	S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester	-	?
S-adenosyl-L-methionine + VYP(iso-D)HA	-	Drosophila melanogaster	S-adenosyl-L-homocysteine + VYP(isoaspartate alpha-methyl ester)HA	-	?
S-adenosyl-L-methionine + VYP(iso-D)HA	-	Drosophila melanogaster	S-adenosyl-L-homocysteine + VYP(L-isoaspartate alpha-methyl ester)HA	-	?

Synonyms

Synonyms	Comment	Organism
PIMT	-	Drosophila melanogaster
protein L-isoaspartyl methyltransferase	-	Drosophila melanogaster

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Release 2023.1 (January 2023)