Literature summary for 2.1.1.63 extracted from

Miggiano, R.; Perugino, G.; Ciaramella, M.; Serpe, M.; Rejman, D.; Pav, O.; Pohl, R.; Garavaglia, S.; Lahiri, S.; Rizzi, M.; Rossi, F.
Crystal structure of Mycobacterium tuberculosis O6-methylguanine-DNA methyltransferase protein clusters assembled onto damaged DNA (2016), Biochem. J., 473, 123-133.

Search for more literature for 2.1.1.63

Crystallization (Commentary)

Crystallization (Comment)	Organism
hanging drop vapor diffusion method, for wild type, enzyme using 0.2 M ammonium acetate, 22% (w/v) PEG 3350, and 0.1 M HEPES pH 7.5, while for mutant enzymes R37K and Y139F using 0.1 M HEPES pH 7.5, 4% polyethylene glycol 8000, and either 4% or 8% ethylene glycol, respectively	Mycobacterium tuberculosis

Protein Variants

Protein Variants	Comment	Organism
R37E	the mutant exhibits a 5fold lower affinity for the methylated duplex DNA compared to the wild type enzyme	Mycobacterium tuberculosis
R37K	the variant performs a sub-optimal alkylated-DNA repair in vitro compared to the wild type enzyme	Mycobacterium tuberculosis
R37L	the variant performs a sub-optimal alkylated-DNA repair in vitro compared to the wild type enzyme	Mycobacterium tuberculosis
Y139F	the mutant exhibits a 10fold lower affinity for the methylated duplex DNA compared to the wild type enzyme	Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
DNA (containing 6-O-methylguanine) + protein L-cysteine	Mycobacterium tuberculosis	-	DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine	-	?

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	P9WJW5	-	-

Purification (Commentary)

Purification (Comment)	Organism
Superdex 200 gel filtration	Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
DNA (containing 6-O-methylguanine) + protein L-cysteine	-	Mycobacterium tuberculosis	DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine	-	?

Synonyms

Synonyms	Comment	Organism
O6-methylguanine-DNA methyltransferase	-	Mycobacterium tuberculosis
OGT	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)