Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O6-methylguanine oligonucleotide | preincubation of extracts with a short oligonucleotide containing a single O6-methylguanine residue causes essentially complete loss of ATase activity | Homo sapiens | |
O6-methylguanine oligonucleotide | preincubation of extracts with a short oligonucleotide containing a single O6-methylguanine residue causes essentially complete loss of ATase activity | Pyrobaculum islandicum | |
O6-methylguanine oligonucleotide | preincubation of extracts with a short oligonucleotide containing a single O6-methylguanine residue causes essentially complete loss of ATase activity | Pyrococcus furiosus | |
O6-methylguanine oligonucleotide | preincubation of extracts with a short oligonucleotide containing a single O6-methylguanine residue causes essentially complete loss of ATase activity | Sulfolobus acidocaldarius | |
O6-methylguanine oligonucleotide | preincubation of extracts with a short oligonucleotide containing a single O6-methylguanine residue causes essentially complete loss of ATase activity | Thermococcus litoralis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
15000 | - |
x * 15000, SDS-PAGE | Pyrobaculum islandicum |
16000 | - |
x * 16000, SDS-PAGE | Sulfolobus acidocaldarius |
22000 | - |
x * 22000, SDS-PAGE | Pyrococcus furiosus |
23000 | - |
x * 23000, SDS-PAGE | Thermococcus litoralis |
24000 | - |
x * 24000, SDS-PAGE | Homo sapiens |
39000 | - |
x * 39000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | Escherichia coli | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | Homo sapiens | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | Pyrococcus furiosus | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | Sulfolobus acidocaldarius | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | Thermococcus litoralis | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | Pyrobaculum islandicum | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
? | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | Escherichia coli BS21 | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Escherichia coli BS21 | - |
- |
- |
Homo sapiens | - |
- |
- |
Pyrobaculum islandicum | - |
- |
- |
Pyrococcus furiosus | - |
- |
- |
Sulfolobus acidocaldarius | - |
- |
- |
Thermococcus litoralis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | - |
Escherichia coli | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | - |
Homo sapiens | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | - |
Pyrococcus furiosus | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | - |
Sulfolobus acidocaldarius | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | - |
Thermococcus litoralis | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | - |
Pyrobaculum islandicum | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Escherichia coli | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Homo sapiens | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Pyrococcus furiosus | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Sulfolobus acidocaldarius | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Thermococcus litoralis | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Pyrobaculum islandicum | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
? | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | - |
Escherichia coli BS21 | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir | |
DNA (containing 6-O-methylguanine) + [protein] L-cysteine | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Escherichia coli BS21 | DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 16000, SDS-PAGE | Sulfolobus acidocaldarius |
? | x * 39000, SDS-PAGE | Escherichia coli |
? | x * 24000, SDS-PAGE | Homo sapiens |
? | x * 23000, SDS-PAGE | Thermococcus litoralis |
? | x * 22000, SDS-PAGE | Pyrococcus furiosus |
? | x * 15000, SDS-PAGE | Pyrobaculum islandicum |
Synonyms | Comment | Organism |
---|---|---|
ATASE | - |
Escherichia coli |
ATASE | - |
Homo sapiens |
ATASE | - |
Pyrococcus furiosus |
ATASE | - |
Sulfolobus acidocaldarius |
ATASE | - |
Thermococcus litoralis |
ATASE | - |
Pyrobaculum islandicum |
O6-alkylguanine-DNA alkyltransferase | - |
Escherichia coli |
O6-alkylguanine-DNA alkyltransferase | - |
Homo sapiens |
O6-alkylguanine-DNA alkyltransferase | - |
Pyrococcus furiosus |
O6-alkylguanine-DNA alkyltransferase | - |
Sulfolobus acidocaldarius |
O6-alkylguanine-DNA alkyltransferase | - |
Thermococcus litoralis |
O6-alkylguanine-DNA alkyltransferase | - |
Pyrobaculum islandicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Escherichia coli |
37 | - |
- |
Homo sapiens |
75 | - |
- |
Sulfolobus acidocaldarius |
90 | - |
- |
Thermococcus litoralis |
100 | - |
- |
Pyrococcus furiosus |
100 | - |
- |
Pyrobaculum islandicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 100 | activity increases from 50°C to 100%, 60°C: about 45% of the activity at 100°C, 50% about 15% of maximal activity | Pyrococcus furiosus |
60 | 100 | activity at 60°C is about 35% of the activity at 100°C | Pyrobaculum islandicum |
65 | 85 | 65°C: about 50% of maximal activity, 85°C: about 45% of maximal activity | Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
t1/2: 32 h | Sulfolobus acidocaldarius |
37 | - |
t1/2: 38 h | Pyrobaculum islandicum |
37 | - |
t1/2: 60 h | Pyrococcus furiosus |
37 | - |
t1/2: 72 h | Thermococcus litoralis |
60 | - |
t1/2: 18 h | Thermococcus litoralis |
60 | - |
t1/2: 12 h | Pyrobaculum islandicum |
60 | - |
t1/2: 16 h | Sulfolobus acidocaldarius |
60 | - |
t1/2: 40 h | Pyrococcus furiosus |
80 | - |
t1/2: 0.5 h | Sulfolobus acidocaldarius |
80 | - |
t1/2: 13 h | Thermococcus litoralis |
80 | - |
t1/2: 5 h | Pyrococcus furiosus |
80 | - |
t1/2: 5.5 h | Pyrobaculum islandicum |
General Information | Comment | Organism |
---|---|---|
physiological function | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Escherichia coli |
physiological function | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Homo sapiens |
physiological function | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Pyrococcus furiosus |
physiological function | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Sulfolobus acidocaldarius |
physiological function | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Thermococcus litoralis |
physiological function | the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein | Pyrobaculum islandicum |