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Literature summary for 2.1.1.63 extracted from

  • Skorvaga, M.; Raven, N.D.; Margison, G.P.
    Thermostable archaeal O6-alkylguanine-DNA alkyltransferases (1998), Proc. Natl. Acad. Sci. USA, 95, 6711-6715.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
O6-methylguanine oligonucleotide preincubation of extracts with a short oligonucleotide containing a single O6-methylguanine residue causes essentially complete loss of ATase activity Homo sapiens
O6-methylguanine oligonucleotide preincubation of extracts with a short oligonucleotide containing a single O6-methylguanine residue causes essentially complete loss of ATase activity Pyrobaculum islandicum
O6-methylguanine oligonucleotide preincubation of extracts with a short oligonucleotide containing a single O6-methylguanine residue causes essentially complete loss of ATase activity Pyrococcus furiosus
O6-methylguanine oligonucleotide preincubation of extracts with a short oligonucleotide containing a single O6-methylguanine residue causes essentially complete loss of ATase activity Sulfolobus acidocaldarius
O6-methylguanine oligonucleotide preincubation of extracts with a short oligonucleotide containing a single O6-methylguanine residue causes essentially complete loss of ATase activity Thermococcus litoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
15000
-
x * 15000, SDS-PAGE Pyrobaculum islandicum
16000
-
x * 16000, SDS-PAGE Sulfolobus acidocaldarius
22000
-
x * 22000, SDS-PAGE Pyrococcus furiosus
23000
-
x * 23000, SDS-PAGE Thermococcus litoralis
24000
-
x * 24000, SDS-PAGE Homo sapiens
39000
-
x * 39000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA (containing 6-O-methylguanine) + [protein] L-cysteine Escherichia coli the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine Homo sapiens the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine Pyrococcus furiosus the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine Sulfolobus acidocaldarius the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine Thermococcus litoralis the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine Pyrobaculum islandicum the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
?
DNA (containing 6-O-methylguanine) + [protein] L-cysteine Escherichia coli BS21 the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli BS21
-
-
-
Homo sapiens
-
-
-
Pyrobaculum islandicum
-
-
-
Pyrococcus furiosus
-
-
-
Sulfolobus acidocaldarius
-
-
-
Thermococcus litoralis
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
-
Escherichia coli DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
-
Homo sapiens DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
-
Pyrococcus furiosus DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
-
Sulfolobus acidocaldarius DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
-
Thermococcus litoralis DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
-
Pyrobaculum islandicum DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Escherichia coli DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Homo sapiens DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Pyrococcus furiosus DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Sulfolobus acidocaldarius DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Thermococcus litoralis DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Pyrobaculum islandicum DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
?
DNA (containing 6-O-methylguanine) + [protein] L-cysteine
-
Escherichia coli BS21 DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir
DNA (containing 6-O-methylguanine) + [protein] L-cysteine the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Escherichia coli BS21 DNA (without 6-O-methylguanine) + [protein] S-methyl-L-cysteine
-
ir

Subunits

Subunits Comment Organism
? x * 16000, SDS-PAGE Sulfolobus acidocaldarius
? x * 39000, SDS-PAGE Escherichia coli
? x * 24000, SDS-PAGE Homo sapiens
? x * 23000, SDS-PAGE Thermococcus litoralis
? x * 22000, SDS-PAGE Pyrococcus furiosus
? x * 15000, SDS-PAGE Pyrobaculum islandicum

Synonyms

Synonyms Comment Organism
ATASE
-
Escherichia coli
ATASE
-
Homo sapiens
ATASE
-
Pyrococcus furiosus
ATASE
-
Sulfolobus acidocaldarius
ATASE
-
Thermococcus litoralis
ATASE
-
Pyrobaculum islandicum
O6-alkylguanine-DNA alkyltransferase
-
Escherichia coli
O6-alkylguanine-DNA alkyltransferase
-
Homo sapiens
O6-alkylguanine-DNA alkyltransferase
-
Pyrococcus furiosus
O6-alkylguanine-DNA alkyltransferase
-
Sulfolobus acidocaldarius
O6-alkylguanine-DNA alkyltransferase
-
Thermococcus litoralis
O6-alkylguanine-DNA alkyltransferase
-
Pyrobaculum islandicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Escherichia coli
37
-
-
Homo sapiens
75
-
-
Sulfolobus acidocaldarius
90
-
-
Thermococcus litoralis
100
-
-
Pyrococcus furiosus
100
-
-
Pyrobaculum islandicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 100 activity increases from 50°C to 100%, 60°C: about 45% of the activity at 100°C, 50% about 15% of maximal activity Pyrococcus furiosus
60 100 activity at 60°C is about 35% of the activity at 100°C Pyrobaculum islandicum
65 85 65°C: about 50% of maximal activity, 85°C: about 45% of maximal activity Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
t1/2: 32 h Sulfolobus acidocaldarius
37
-
t1/2: 38 h Pyrobaculum islandicum
37
-
t1/2: 60 h Pyrococcus furiosus
37
-
t1/2: 72 h Thermococcus litoralis
60
-
t1/2: 18 h Thermococcus litoralis
60
-
t1/2: 12 h Pyrobaculum islandicum
60
-
t1/2: 16 h Sulfolobus acidocaldarius
60
-
t1/2: 40 h Pyrococcus furiosus
80
-
t1/2: 0.5 h Sulfolobus acidocaldarius
80
-
t1/2: 13 h Thermococcus litoralis
80
-
t1/2: 5 h Pyrococcus furiosus
80
-
t1/2: 5.5 h Pyrobaculum islandicum

General Information

General Information Comment Organism
physiological function the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Escherichia coli
physiological function the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Homo sapiens
physiological function the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Pyrococcus furiosus
physiological function the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Sulfolobus acidocaldarius
physiological function the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Thermococcus litoralis
physiological function the DNA repair protein O6-alkylguanine-DNA alkyltransferase is a principal mechanism of cellular resistance to the toxic and mutagenic effects of DNA damage produced by certain monofunctional alkylating agents. ATase operates by the transfer of the offending alkyl groups from the O6 position of guanine and the O4 position of thymine in damaged DNA to a cysteine residue at the active site of the protein. This is an irreversible process that results in the stoichiometric inactivation of the protein Pyrobaculum islandicum