Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the H5 variant harbouring five mutations (in the helix-turn helix motif) has a catalytic activity on O-6-benzyl guanine derivated substrates, whereas its ability to bind and repair the natural DNA containing 6-O-methylguanine is completely abolished | Saccharolobus solfataricus |
General Stability | Organism |
---|---|
very stable to proteases attack and detergents such as Triton X-100 | Saccharolobus solfataricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-O-benzylguanine | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00077 | - |
DNA (containing 6-O-methylguanine) | pH 6.5, 50°C | Saccharolobus solfataricus | |
0.01 | - |
SNAP-Vista Green | pH 6.5, 50°C | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Saccharolobus solfataricus | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17040 | - |
amino acid sequence | Saccharolobus solfataricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine | Saccharolobus solfataricus | - |
DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine | - |
? | |
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine | Saccharolobus solfataricus P2 | - |
DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97VW7 | - |
- |
Saccharolobus solfataricus P2 | Q97VW7 | - |
- |
Purification (Comment) | Organism |
---|---|
heterologous expression by His-tag chromatography affinity | Saccharolobus solfataricus |
Storage Stability | Organism |
---|---|
-20°C stable for years | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine | - |
Saccharolobus solfataricus | DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine | - |
? | |
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine | - |
Saccharolobus solfataricus P2 | DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine | - |
? | |
SNAP-Vista Green + [protein]-L-cysteine | fluorescent 6-O-benzylguanine derivative | Saccharolobus solfataricus | guanine + [protein]-S-Vista Green-L-cysteine | - |
? | |
SNAP-Vista Green + [protein]-L-cysteine | fluorescent 6-O-benzylguanine derivative | Saccharolobus solfataricus P2 | guanine + [protein]-S-Vista Green-L-cysteine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | - |
Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
AGT | - |
Saccharolobus solfataricus |
MGMT | - |
Saccharolobus solfataricus |
OGT | - |
Saccharolobus solfataricus |
SSO2487 | locus name | Saccharolobus solfataricus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 90 | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
half life of 257 min | Saccharolobus solfataricus |
70 | - |
half life of 165 min | Saccharolobus solfataricus |
80 | - |
half life of 19 min | Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
8.4 | - |
SNAP-Vista Green | pH 6.5, 50°C | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Saccharolobus solfataricus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 9.5 | - |
Saccharolobus solfataricus |