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Literature summary for 2.1.1.63 extracted from

  • Perugino, G.; Vettone, A.; Illiano, G.; Valenti, A.; Ferrara, M.C.; Rossi, M.; Ciaramella, M.
    Activity and regulation of archaeal DNA alkyltransferase: conserved protein involved in repair of DNA alkylation damage (2012), J. Biol. Chem., 287, 4222-4231.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
additional information the H5 variant harbouring five mutations (in the helix-turn helix motif) has a catalytic activity on O-6-benzyl guanine derivated substrates, whereas its ability to bind and repair the natural DNA containing 6-O-methylguanine is completely abolished Saccharolobus solfataricus

General Stability

General Stability Organism
very stable to proteases attack and detergents such as Triton X-100 Saccharolobus solfataricus

Inhibitors

Inhibitors Comment Organism Structure
6-O-benzylguanine
-
Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00077
-
DNA (containing 6-O-methylguanine) pH 6.5, 50°C Saccharolobus solfataricus
0.01
-
SNAP-Vista Green pH 6.5, 50°C Saccharolobus solfataricus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Saccharolobus solfataricus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17040
-
amino acid sequence Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine Saccharolobus solfataricus
-
DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine
-
?
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine Saccharolobus solfataricus P2
-
DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97VW7
-
-
Saccharolobus solfataricus P2 Q97VW7
-
-

Purification (Commentary)

Purification (Comment) Organism
heterologous expression by His-tag chromatography affinity Saccharolobus solfataricus

Storage Stability

Storage Stability Organism
-20°C stable for years Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine
-
Saccharolobus solfataricus DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine
-
?
DNA (containing 6-O-methylguanine) + [protein]-L-cysteine
-
Saccharolobus solfataricus P2 DNA (without 6-O-methylguanine) + [protein]-S-methyl-L-cysteine
-
?
SNAP-Vista Green + [protein]-L-cysteine fluorescent 6-O-benzylguanine derivative Saccharolobus solfataricus guanine + [protein]-S-Vista Green-L-cysteine
-
?
SNAP-Vista Green + [protein]-L-cysteine fluorescent 6-O-benzylguanine derivative Saccharolobus solfataricus P2 guanine + [protein]-S-Vista Green-L-cysteine
-
?

Subunits

Subunits Comment Organism
monomer
-
Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
AGT
-
Saccharolobus solfataricus
MGMT
-
Saccharolobus solfataricus
OGT
-
Saccharolobus solfataricus
SSO2487 locus name Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 90
-
Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
half life of 257 min Saccharolobus solfataricus
70
-
half life of 165 min Saccharolobus solfataricus
80
-
half life of 19 min Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
8.4
-
SNAP-Vista Green pH 6.5, 50°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 9.5
-
Saccharolobus solfataricus