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Literature summary for 2.1.1.63 extracted from
- The L84F polymorphic variant of human O6-methylguanine-DNA methyltransferase alters stability in U87MG glioma cells but not temozolomide sensitivity (2009), Neuro-oncology, 11, 22-32.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in HEK-293T cells as enhanced green fluorescent protein-tagged enzyme | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| I143V/K178R | enhanced green fluorescent protein-tagged MGMT variants exhibit nuclear localization patterns indistinguishable from wild type enzyme | Homo sapiens |
| L84F | the L84F polymorphic variant of human O6-methylguanine-DNA methyltransferase alters stability in U87MG glioma cells but not temozolomide sensitivity, upon exposure to O6-benzylguanine, the L84F variant is degraded more rapidly than wild type | Homo sapiens |
| L84F/I143V/K178R | enhanced green fluorescent protein-tagged MGMT variants exhibit nuclear localization patterns indistinguishable from wild type enzyme, upon exposure to O6-benzylguanine, the L84F/I143V/K178R variant is degraded more rapidly than wild type | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| O6-benzylguanine | a synthetic MGMT inhibitor | Homo sapiens |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Homo sapiens | 5634 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
enhanced green fluorescent protein-tagged enzyme, SDS-PAGE | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| glioma cell | - |
Homo sapiens | - |
| U-87MG cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| DNA containing 6-O-methylguanine + [protein]-L-cysteine | - |
Homo sapiens | DNA lacking 6-O-methylguanine + [protein]-S-methyl-L-cysteine | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MGMT | - |
Homo sapiens |
| O6-methylguanine-DNA methyltransferase | - |
Homo sapiens |
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Release 2023.1 (January 2023)
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