Cloned (Comment) | Organism |
---|---|
recombinant variants are expressed in HMS174 pLyS cells | Thermococcus kodakarensis |
Protein Variants | Comment | Organism |
---|---|---|
E158A | melting temperature of mutant enzyme E83A at 5 mM urea is 90.6°C, compared to 91.5°C for the wild-type enzyme | Thermococcus kodakarensis |
E159A | melting temperature of mutant enzyme E83A at 5 mM urea is 91.7°C, compared to 91.5°C for the wild-type enzyme | Thermococcus kodakarensis |
E83A | melting temperature of mutant enzyme E83A at 5 mM urea is 89.2°C, compared to 91.5°C for the wild-type enzyme | Thermococcus kodakarensis |
E93A | mutant enzyme unfolds one order of magnitude faster than does the wild-type enzyme | Thermococcus kodakarensis |
Organic Solvent | Comment | Organism |
---|---|---|
urea | 5 mM, melting temperature of wild-type enzyme: 91.5 °C, melting temperature of mutant enzyme E83A: 89.2°C, melting temperature of mutant enzyme E93A: 85.5°C, melting temperature of mutant enzyme E158A: 90.6°C, melting temperature of mutant enzyme E159A: 91.7 °C | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
O6-methylguanine-DNA methyltransferase | - |
Thermococcus kodakarensis |
Tk-MGMT | - |
Thermococcus kodakarensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Thermococcus kodakarensis |